Crystal structure of the ligand-binding domain of the promiscuous EphA4 receptor reveals two distinct conformations Journal Article


Authors: Singla, N.; Goldgur, Y.; Xu, K.; Paavilainen, S.; Nikolov, D. B.; Himanen, J. P.
Article Title: Crystal structure of the ligand-binding domain of the promiscuous EphA4 receptor reveals two distinct conformations
Abstract: Eph receptors and their ephrin ligands are important mediators of cell-cell communication. They are divided in two subclasses based on their affinities for each other and on sequence conservation. Receptor-ligand binding within each subclass is fairly promiscuous, while binding cross the subclasses happens rarely. EphA4 is an exception to this general rule, since it has long been known to bind both A- and B-class ephrin ligands but the reason for this exceptional behavior has not been worked out at molecular level. Recent structural and biochemical studies on EphA4 ligand-binding domain alone and in complex with its ligands have addressed this question. However, the published structures of EphA4/ephrin complexes differ considerably from each other and strikingly different explanations for the exceptional promiscuity of EphA4 were proposed. To address these contradictory findings, we have determined a crystal structure of the EphA4 ligand-binding domain at 2.3. Å resolution and show that the receptor has an unprecedented ability to exist in two very different, well-ordered conformations even in the unbound state. Our results suggest that the ligand promiscuity of the Ephs is directly correlated with the structural flexibility of the ligand-binding surface of the receptor. © 2010 Elsevier Inc.
Keywords: protein binding; receptor tyrosine kinase; ligands; crystal structure; crystallography, x-ray; protein structure, tertiary; ephrin receptor a2; conformational transition; conformation; protein structure; ligand binding; ephrin receptor a1; x-ray crystallography; ephrin; eph; ephrin b2; loop-flexibility; ephrin receptor a4; receptor, epha4
Journal Title: Biochemical and Biophysical Research Communications
Volume: 399
Issue: 4
ISSN: 0006-291X
Publisher: Elsevier Science, Inc.  
Date Published: 2010-09-03
Start Page: 555
End Page: 559
Language: English
DOI: 10.1016/j.bbrc.2010.07.109
PUBMED: 20678482
PROVIDER: scopus
PMCID: PMC2949057
DOI/URL:
Notes: --- - "Export Date: 20 April 2011" - "CODEN: BBRCA" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Dimitar B Nikolov
    70 Nikolov
  2. Juha P Himanen
    42 Himanen
  3. Yehuda Goldgur
    24 Goldgur
  4. Nikhil Singla
    3 Singla
  5. Kai Xu
    18 Xu