In vivo and in vitro models of ionizing radiation tothe vocal folds Journal Article
| Authors: | Saltman, B.; Kraus, D. H.; Szeto, H.; Parashar, B.; Ghossein, R.; Felsen, D.; Branski, R. C. |
| Article Title: | In vivo and in vitro models of ionizing radiation tothe vocal folds |
| Abstract: | Background. Radiation therapy (RT) to the head and neck often results in damage to the vocal folds (VF) and surrounding structures. Characterization and treatment of these sequelae is limited, likely due to the lack of experimental models. Methods. Larynges from rats exposed to 2 fractionation schedules (40 Gy total) were analyzed histologically. In vitro, reactive oxygen species (ROS) synthesis, and transcription of select genes associated with ROS, inflammation, and fibrosis were examined in VF fibroblasts after single-dose radiation. Results. Although radiation-induced histologic alterations are made to VF architecture, 1 fractionation schedule was accompanied by significant morbidity and mortality. In vitro, radiation increased ROS synthesis and inflammatory and profibrotic gene expression. Conclusion. Our data suggest that hyperfractionated RT is more tolerable. Utilizing this model, RT-induced histologic aberrations are made to the VF mucosa. In addition, a relationship between radiation, ROS, and inflammatory and fibrotic gene expression was observed in vitro. © 2009 Wiley Periodicals, Inc. |
| Keywords: | controlled study; human cell; mortality; vocal cord; nonhuman; animals; animal tissue; gene expression; radiation; transforming growth factor beta; dehydration; morbidity; animal experiment; weight loss; genetic transcription; in vivo study; in vitro study; histology; fibrosis; models, animal; radiation dose fractionation; rna, messenger; rat; erythema; vocal fold; voice; fibroblast; vocal cords; reactive oxygen species; reactive oxygen metabolite; heme oxygenase-1; laryngeal mucosa; ionizing radiation; rats; rats, sprague-dawley; dose fractionation; alopecia; larynx; skin ulcer; matrix metalloproteinase 1; hypertrophy |
| Journal Title: | Head & Neck |
| Volume: | 32 |
| Issue: | 5 |
| ISSN: | 1043-3074 |
| Publisher: | John Wiley & Sons, Inc. |
| Date Published: | 2010-05-01 |
| Start Page: | 572 |
| End Page: | 577 |
| Language: | English |
| DOI: | 10.1002/hed.21212 |
| PUBMED: | 19672960 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 20 April 2011" - "CODEN: HEANE" - "Source: Scopus" |
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