Protocol for PTEN expression by immunohistochemistry in formalin-fixed paraffin-embedded human breast carcinoma Journal Article

   


Authors: Sakr, R.; Barbashina, V.; Morrogh, M.; Chandarlapaty, S.; Andrade, V. P.; Arroyo, C. D.; Olvera, N.; King, T. A.
Article Title: Protocol for PTEN expression by immunohistochemistry in formalin-fixed paraffin-embedded human breast carcinoma
Abstract: The PI3K/PTEN pathway plays a major role in carcinogenesis. Dysregulation of this pathway occurs frequently in breast cancer, and loss of PTEN expression is emerging as a potentially important mechanism of resistance to the widely used anti-HER2 therapy, trastuzumab. However, assays for loss of PTEN expression have suffered from lack of consistency. Here, we describe an automated and reliable protocol for PTEN protein expression by immunohistochemistry in formalin-fixed paraffin-embedded tissue that can be easily incorporated into clinical trials. © 2010 by Lippincott Williams & Wilkins.
Keywords: immunohistochemistry; controlled study; human tissue; protein expression; human cell; protein localization; reproducibility of results; breast cancer; observer variation; practice guideline; high throughput screening; cell line, tumor; breast neoplasms; automation; time factors; blotting, western; quantitative analysis; breast carcinoma; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; pten phosphohydrolase; scoring system; carcinoma; formaldehyde; paraffin embedding; automated protocol; pten protein expression
Journal Title: Applied Immunohistochemistry & Molecular Morphology
Volume: 18
Issue: 4
ISSN: 1541-2016
Publisher: Lippincott Williams & Wilkins  
Date Published: 2010-07-01
Start Page: 371
End Page: 374
Language: English
DOI: 10.1097/PAI.0b013e3181d50bd5
PUBMED: 20216404
PROVIDER: scopus
PMCID: PMC2921801
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-77954144291&partnerID=40&md5=4129a7dc88b80ecdb703b7cbc53924a8
Notes: --- - "Cited By (since 1996): 1" - "Export Date: 20 April 2011" - "CODEN: AIMMF" - "Source: Scopus"
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MSK Authors
  1. Violetta Barbashina
    30 Barbashina
  2. Tari King
    186 King
  3. Sarat Chandarlapaty
    280 Chandarlapaty
  4. Mary Morrogh
    33 Morrogh
  5. Rita Sakr
    61 Sakr
  6. Victor Piana De Andrade
    27 Andrade
  7. Crispinita D Arroyo
    30 Arroyo
  8. Narciso Olvera
    73 Olvera
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