Authors: | Sakr, R.; Barbashina, V.; Morrogh, M.; Chandarlapaty, S.; Andrade, V. P.; Arroyo, C. D.; Olvera, N.; King, T. A. |
Article Title: | Protocol for PTEN expression by immunohistochemistry in formalin-fixed paraffin-embedded human breast carcinoma |
Abstract: | The PI3K/PTEN pathway plays a major role in carcinogenesis. Dysregulation of this pathway occurs frequently in breast cancer, and loss of PTEN expression is emerging as a potentially important mechanism of resistance to the widely used anti-HER2 therapy, trastuzumab. However, assays for loss of PTEN expression have suffered from lack of consistency. Here, we describe an automated and reliable protocol for PTEN protein expression by immunohistochemistry in formalin-fixed paraffin-embedded tissue that can be easily incorporated into clinical trials. © 2010 by Lippincott Williams & Wilkins. |
Keywords: | immunohistochemistry; controlled study; human tissue; protein expression; human cell; protein localization; reproducibility of results; breast cancer; observer variation; practice guideline; high throughput screening; cell line, tumor; breast neoplasms; automation; time factors; blotting, western; quantitative analysis; breast carcinoma; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; pten phosphohydrolase; scoring system; carcinoma; formaldehyde; paraffin embedding; automated protocol; pten protein expression |
Journal Title: | Applied Immunohistochemistry & Molecular Morphology |
Volume: | 18 |
Issue: | 4 |
ISSN: | 1541-2016 |
Publisher: | Lippincott Williams & Wilkins |
Date Published: | 2010-07-01 |
Start Page: | 371 |
End Page: | 374 |
Language: | English |
DOI: | 10.1097/PAI.0b013e3181d50bd5 |
PUBMED: | 20216404 |
PROVIDER: | scopus |
PMCID: | PMC2921801 |
DOI/URL: | |
Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 20 April 2011" - "CODEN: AIMMF" - "Source: Scopus" |