Synthesis of granulocyte-macrophage colony-stimulating factor as homogeneous glycoforms and early comparisons with yeast cell-derived material Journal Article

   

Authors:	Zhang, Q.; Johnston, E. V.; Shieh, J.H.; Moore, M. A. S.; Danishefsky, S. J.
Article Title:	Synthesis of granulocyte-macrophage colony-stimulating factor as homogeneous glycoforms and early comparisons with yeast cell-derived material
Abstract:	Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a medicinally important glycoprotein, used as an immunostimulant following bone-marrow transplant. On the basis of reports of its potential utility as an anticancer vaccine adjuvant, we undertook to develop a synthetic route toward single-glycoform GM-CSF. We describe herein a convergent total synthesis of GM-CSF aglycone and two homogeneous glycoforms. Analytical and biological studies confirm the structure and activity of these synthetic congeners.
Keywords:	nonhuman; granulocyte macrophage colony stimulating factor; protein structure; yeast cell; peptide synthesis; glycoprotein synthesis; protein glycosylation; alanine ligation; aglycone; priority journal; article; peptide ligation
Journal Title:	Proceedings of the National Academy of Sciences of the United States of America
Volume:	111
Issue:	8
ISSN:	0027-8424
Publisher:	National Academy of Sciences
Date Published:	2014-02-25
Start Page:	2885
End Page:	2890
Language:	English
DOI:	10.1073/pnas.1400140111
PROVIDER:	scopus
PMCID:	PMC3939888
PUBMED:	24516138
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84896823976&partnerID=40&md5=aa92ca822f60d1156360ab51143a4b0b
Notes:	Export Date: 2 April 2014 -- CODEN: PNASA -- Source: Scopus

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