Modeling alzheimer's disease in mouse without mutant protein overexpression: Cooperative and independent effects of Aβ and tau Journal Article

Authors: Guo, Q.; Li, H.; Cole, A. L.; Hur, J. Y.; Li, Y.; Zheng, H.
Article Title: Modeling alzheimer's disease in mouse without mutant protein overexpression: Cooperative and independent effects of Aβ and tau
Abstract: Background: Alzheimer's disease (AD), the most common cause of dementia in the elderly, has two pathological hallmarks: Aβ plaques and aggregation of hyperphosphorylated tau (p-tau). Aβ is a cleavage product of Amyloid Precursor Protein (APP). Presenilin 1 (PS1) and presenilin 2 (PS2) are the catalytic subunit of γ-secretase, which cleaves APP and mediates Aβ production. Genetic mutations in APP, PSEN1 or PSEN2 can lead to early onset of familial AD (FAD). Although mutations in the tau encoding gene MAPT leads to a subtype of frontotemporal dementia and these mutations have been used to model AD tauopathy, no MAPT mutations have been found to be associated with AD. Results: To model AD pathophysiology in mice without the gross overexpression of mutant transgenes, we created a humanized AD mouse model by crossing the APP and PSEN1 FAD knock-in mice with the htau mice which express wildtype human MAPT genomic DNA on mouse MAPT null background (APP/PS1/htau). The APP/PS1/htau mice displayed mild, age-dependent, Aβ plaques and tau hyperphosphorylation, thus successfully recapitulating the late-onset AD pathological hallmarks. Selected biochemical analyses, including p-tau western blot, γ-secretase activity assay, and Aβ ELISA, were performed to study the interaction between Aβ and p-tau. Subsequent behavioral studies revealed that the APP/PS1/htau mice showed reduced mobility in old ages and exaggerated fear response. Genetic analysis suggested that the fear phenotype is due to a synergic interaction between Aβ and p-tau, and it can be completely abolished by tau deletion. Conclusion: The APP/PS1/htau model represents a valuable and disease-relevant late-onset pre-clinical AD animal model because it incorporates human AD genetics without mutant protein overexpression. Analysis of the mice revealed both cooperative and independent effects of Aβ and p-tau. © 2013 Guo et al.
Keywords: protein expression; protein phosphorylation; gene deletion; nonhuman; genetic analysis; mouse; phenotype; animal tissue; gene overexpression; protein protein interaction; animal experiment; animal model; enzyme activity; enzyme linked immunosorbent assay; disease model; western blotting; transgene; gene inactivation; fear; alzheimer disease; genomic dna; neuropathology; amyloid precursor protein; gamma secretase; presenilin 1; tau protein; mouse model; amyloid beta protein; male; article; amyloid plaque; app gene; mapt gene; psen1 gene
Journal Title: PLoS ONE
Volume: 8
Issue: 11
ISSN: 1932-6203
Publisher: Public Library of Science  
Date Published: 2013-11-20
Start Page: e80706
Language: English
DOI: 10.1371/journal.pone.0080706
PROVIDER: scopus
PMCID: PMC3835479
PUBMED: 24278307
Notes: Export Date: 2 April 2014 -- CODEN: POLNC -- Source: Scopus
Citation Impact
MSK Authors
  1. Yueming Li
    107 Li
  2. Ji Yeun Hur
    4 Hur