Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis Journal Article
| Authors: | Stroud, H.; Do, T.; Du, J.; Zhong, X.; Feng, S.; Johnson, L.; Patel, D. J.; Jacobsen, S. E. |
| Article Title: | Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis |
| Abstract: | DNA methylation occurs in CG and non-CG sequence contexts. Non-CG methylation is abundant in plants and is mediated by CHROMOMETHYLASE (CMT) and DOMAINS REARRANGED METHYLTRANSFERASE (DRM) proteins; however, its roles remain poorly understood. Here we characterize the roles of non-CG methylation in Arabidopsis thaliana. We show that a poorly characterized methyltransferase, CMT2, is a functional methyltransferase in vitro and in vivo. CMT2 preferentially binds histone H3 Lys9 (H3K9) dimethylation and methylates non-CG cytosines that are regulated by H3K9 methylation. We revealed the contributions and redundancies between each non-CG methyltransferase in DNA methylation patterning and in regulating transcription. We also demonstrate extensive dependencies of small-RNA accumulation and H3K9 methylation patterning on non-CG methylation, suggesting self-reinforcing mechanisms between these epigenetic factors. The results suggest that non-CG methylation patterns are critical in shaping the landscapes of histone modification and small noncoding RNA. © 2014 Nature America, Inc. All rights reserved. |
| Journal Title: | Nature Structural and Molecular Biology |
| Volume: | 21 |
| Issue: | 1 |
| ISSN: | 1545-9993 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2014-01-01 |
| Start Page: | 64 |
| End Page: | 72 |
| Language: | English |
| DOI: | 10.1038/nsmb.2735 |
| PROVIDER: | scopus |
| PUBMED: | 24336224 |
| PMCID: | PMC4103798 |
| DOI/URL: | |
| Notes: | Export Date: 3 March 2014 -- CODEN: NSMBC -- Source: Scopus |
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