Annexin 2 is not required for human immunodeficiency virus type 1 particle production but plays a cell type-dependent role in regulating infectivity Journal Article
| Authors: | Rai, T.; Mosoian, A.; Resh, M. D. |
| Article Title: | Annexin 2 is not required for human immunodeficiency virus type 1 particle production but plays a cell type-dependent role in regulating infectivity |
| Abstract: | During assembly and budding of retroviruses, host cell proteins are incorporated into viral particles. Identification of virion-associated proteins may help pinpoint key cellular components required for virus production and function. The cellular protein annexin 2 (Anx2) is incorporated into HIV-1 particles, and knockdown of Anx2 has been reported to cause defects in Gag processing and infectivity of HIV-1 particles in macrophages. Here, we tested whether Anx2 was required for HIV-1 production in other cell types capable of producing HIV-1 virions. Endogenous Anx2 levels were knocked down by ∼98% using lentivirus encoding short hairpin RNAs (shRNAs) or small interfering RNAs (siRNAs) targeting Anx2. Under these conditions, there was no reduction in HIV-1 virus-like particle (VLP) production in either COS-1, 293T, or Jurkat T cells or primary human monocyte-derived macrophages (MDMs). Murine embryonic fibroblasts derived from Anx2-/- mice produced the same levels of VLPs as matched cells from wild-type mice. The calcium-mediated spike in VLP production still occurred in Anx2-depleted COS-1 cells, and there was no apparent alteration in the intracellular Gag localization. Overexpression of Anx2 in trans had no effect on Gag processing or VLP production. Neither Anx2 depletion nor Anx2 overexpression altered the infectivity of HIV-1 particles produced by COS-1 or 293T cells. However, supernatants containing virus from Anx2 siRNA-treated primary human MDMs exhibited decreased infectivity. These data indicate that Anx2 is not required for HIV-1 assembly or Gag processing but rather plays a cell type-dependent role in regulating production of infectious HIV-1 by macrophages. Copyright © 2010, American Society for Microbiology. All Rights Reserved. |
| Keywords: | controlled study; human cell; nonhuman; mouse; animals; mice; mice, knockout; actin; cells, cultured; mus; cell protein; cell line; animal experiment; small interfering rna; rna, small interfering; receptor, epidermal growth factor; immunofluorescence; cercopithecus aethiops; cos cells; cell type; cell strain hek293; western blotting; cell isolation; lentivirus; murinae; fibroblast; cellular distribution; base sequence; monocyte; macrophage; macrophages; lipocortin 2; short hairpin rna; gene knockdown techniques; host cell; human immunodeficiency virus 1; leukemia cell line; jurkat cells; hiv-1; s100 proteins; virus assembly; cell strain u937; supernatant; gag protein; virion; virus infectivity; virus like agent; annexin a2; gag gene products, human immunodeficiency virus; host-pathogen interactions; virulence; virus release |
| Journal Title: | Journal of Virology |
| Volume: | 84 |
| Issue: | 19 |
| ISSN: | 0022-538X |
| Publisher: | American Society for Microbiology |
| Date Published: | 2010-10-01 |
| Start Page: | 9783 |
| End Page: | 9792 |
| Language: | English |
| DOI: | 10.1128/jvi.01584-09 |
| PUBMED: | 20631122 |
| PROVIDER: | scopus |
| PMCID: | PMC2937750 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 20 April 2011" - "CODEN: JOVIA" - "Source: Scopus" |
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