Cleavage of TFIIA by taspase1 activates TRF2-specified mammalian male germ cell programs Journal Article
| Authors: | Oyama, T.; Sasagawa, S.; Takeda, S.; Hess, R. A.; Lieberman, P. M.; Cheng, E. H.; Hsieh, J. J. |
| Article Title: | Cleavage of TFIIA by taspase1 activates TRF2-specified mammalian male germ cell programs |
| Abstract: | The evolution of tissue-specific general transcription factors (GTFs), such as testis-specific TBP-related factor 2 (TRF2), enables the spatiotemporal expression of highly specialized genetic programs. Taspase1 is a protease that cleaves nuclear factors MLL1, MLL2, TFIIAα-β, and ALFα-β (TFIIAτ). Here, we demonstrate that Taspase1-mediated processing of TFIIAα-β drives mammalian spermatogenesis. Both Taspase1-/- and noncleavable TFIIAα-βnc/nc testes release immature germ cells with impaired transcription of Transition proteins (Tnp) and Protamines (Prm), exhibiting chromatin compaction defects and recapitulating those observed with TRF2-/- testes. Although the unprocessed TFIIA still complexes with TRF2, this complex is impaired in targeting and thus activating Tnp1 and Prm1 promoters. The current study presents a paradigm in which a protease (Taspase1) cleaves a ubiquitously expressed GTF (TFIIA) to enable tissue-specific(testis) transcription, meeting the demand for sophisticated regulation of distinct subsets of genes in higher organisms. © 2013 Elsevier Inc. |
| Keywords: | controlled study; protein expression; unclassified drug; promoter region; nonhuman; animal cell; mouse; animal tissue; germ cell; protein targeting; animal experiment; genetic transcription; telomeric repeat binding factor 2; ubiquitination; chromatin; mammal; proteinase; testis; protein cleavage; spermatogenesis; protamine; taspase1; transcription factor iia; transcription factor iiaalpha beta |
| Journal Title: | Developmental Cell |
| Volume: | 27 |
| Issue: | 2 |
| ISSN: | 1534-5807 |
| Publisher: | Cell Press |
| Date Published: | 2013-10-28 |
| Start Page: | 188 |
| End Page: | 200 |
| Language: | English |
| DOI: | 10.1016/j.devcel.2013.09.025 |
| PROVIDER: | scopus |
| PUBMED: | 24176642 |
| PMCID: | PMC3947863 |
| DOI/URL: | |
| Notes: | --- - Cited By (since 1996):1 - "Export Date: 2 December 2013" - "CODEN: DCEEB" - "Source: Scopus" |
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