Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap Journal Article

   

Authors:	Curanovic, D.; Cohen, M.; Singh, I.; Slagle, C. E.; Leslie, C. S.; Jaffrey, S. R.
Article Title:	Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap
Abstract:	Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of transcripts in cells. Next-generation sequencing of labeled mRNAs enables a transcriptome-wide profile of polyadenylation and provides insights into the mRNA sequence elements that are correlated with polyadenylation. © 2013 Nature America, Inc. All rights reserved.
Keywords:	controlled study; gene sequence; human cell; nonhuman; animal cell; stimulus; nucleoside analog; messenger rna; cell stimulation; click chemistry; xenopus laevis; polyadenylation; oocyte maturation; polyadenylic acid; adenosine derivative; germinal vesicle
Journal Title:	Nature Chemical Biology
Volume:	9
Issue:	11
ISSN:	1552-4450
Publisher:	Nature Publishing Group
Date Published:	2013-11-01
Start Page:	671
End Page:	673
Language:	English
DOI:	10.1038/nchembio.1334
PROVIDER:	scopus
PMCID:	PMC3805764
PUBMED:	23995769
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84886598801&partnerID=40&md5=00373e9be51ac02123032cbfedbd26ac
Notes:	--- - "Export Date: 2 December 2013" - "CODEN: NCBAB" - "Source: Scopus"

https://synapse.mskcc.org/synapse/works/has_related_data_chk/47458