Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap Journal Article


Authors: Curanovic, D.; Cohen, M.; Singh, I.; Slagle, C. E.; Leslie, C. S.; Jaffrey, S. R.
Article Title: Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap
Abstract: Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of transcripts in cells. Next-generation sequencing of labeled mRNAs enables a transcriptome-wide profile of polyadenylation and provides insights into the mRNA sequence elements that are correlated with polyadenylation. © 2013 Nature America, Inc. All rights reserved.
Keywords: controlled study; gene sequence; human cell; nonhuman; animal cell; stimulus; nucleoside analog; messenger rna; cell stimulation; click chemistry; xenopus laevis; polyadenylation; oocyte maturation; polyadenylic acid; adenosine derivative; germinal vesicle
Journal Title: Nature Chemical Biology
Volume: 9
Issue: 11
ISSN: 1552-4450
Publisher: Nature Publishing Group  
Date Published: 2013-11-01
Start Page: 671
End Page: 673
Language: English
DOI: 10.1038/nchembio.1334
PROVIDER: scopus
PMCID: PMC3805764
PUBMED: 23995769
DOI/URL:
Notes: --- - "Export Date: 2 December 2013" - "CODEN: NCBAB" - "Source: Scopus"
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MSK Authors
  1. Christina Leslie
    106 Leslie
  2. Irtisha Singh
    8 Singh