Progenitor stem cell marker expression by pulmonary carcinomas Journal Article
| Authors: | Moreira, A. L.; Gonen, M.; Rekhtman, N.; Downey, R. J. |
| Article Title: | Progenitor stem cell marker expression by pulmonary carcinomas |
| Abstract: | Carcinomas may arise as a disorder of regeneration, so that a malignant cell may represent a failure to fully attain the characteristics of differentiated tissue. We hypothesized that there is a differential distribution of progenitor cell markers among different histological types of lung cancers, with poorly differentiated tumors being more likely to express progenitor stem cell markers. The study was limited to paraffin-embedded archival material of resected untreated pulmonary carcinomas, including adenocarcinoma, squamous cell carcinoma, large cell carcinoma, and small cell carcinoma. The sections were stained for putative stem cells markers (Musashi-1, Musashi-2, CD34, CD21, KIT, CD133, p63, and OCT-4). Positivity was read as isolated, focal, or diffuse staining. Stem cell markers were detected in all histological types of pulmonary carcinomas. There was a difference in the expression of markers among the histological types. Small cell carcinoma showed diffuse positivity for most of the markers; in contrast to focal or negative staining in other histological groups. An inverse relationship between CD21 and Musashi-1 was observed. No staining for OCT-4 and CD34 was seen in any of the tumor types. Hierarchical clustering based on marker expression separated tumors into two groups, with one group marked by high expression of Musashi-1 and KIT, contained most of the poorly differentiated adenocarcinomas and small cell carcinomas. Therefore, stem cell markers are expressed in lung cancers with different patterns seen for different histological types and degrees of differentiation. © 2010 USCAP, Inc. All rights reserved. |
| Keywords: | immunohistochemistry; human tissue; protein expression; aged; middle aged; cancer surgery; unclassified drug; major clinical study; carcinoma, squamous cell; adenocarcinoma; cd34 antigen; stem cell factor; proto-oncogene proteins c-kit; cluster analysis; lung neoplasms; tumor markers, biological; nerve tissue proteins; cell differentiation; histology; rna-binding proteins; lung small cell cancer; lung adenocarcinoma; neoplastic stem cells; cell surface marker; tumor suppressor proteins; carcinoma; lung; peptides; lung carcinoma; protein p63; trans-activators; cd133 antigen; antigens, cd; large cell carcinoma; lung squamous cell carcinoma; cell marker; lung surgery; antigens, cd34; glycoproteins; octamer transcription factor 4; octamer transcription factor-3; tissue differentiation; differentiation; paraffin; carcinoma, small cell; hypothesis; paraffin embedding; carcinoma, large cell; complement component c3d receptor; musashi 1 protein; musashi 2 protein; stem cell marker; isolation procedure; receptors, complement 3d |
| Journal Title: | Modern Pathology |
| Volume: | 23 |
| Issue: | 6 |
| ISSN: | 0893-3952 |
| Publisher: | Nature Research |
| Date Published: | 2010-06-01 |
| Start Page: | 889 |
| End Page: | 895 |
| Language: | English |
| DOI: | 10.1038/modpathol.2010.68 |
| PUBMED: | 20305619 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 4" - "Export Date: 20 April 2011" - "CODEN: MODPE" - "Source: Scopus" |
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