Cyclin C regulates human hematopoietic stem/progenitor sell quiescence Journal Article
| Authors: | Miyata, Y.; Liu, Y.; Jankovic, V.; Sashida, G.; Lee, J. M.; Shieh, J.H.; Naoe, T.; Moore, M.; Nimer, S. D. |
| Article Title: | Cyclin C regulates human hematopoietic stem/progenitor sell quiescence |
| Abstract: | Hematopoietic stem cells (HSCs) can remain quiescent or they can enter the cell cycle, and either self-renew or differentiate. Although cyclin C and cyclin dependent kinase (cdk3) are essential for the transition from the G0 to the G1 phase of the cell cycle in human fibroblasts, the role of cyclin C in hematopoietic stem/progenitor cells (HSPCs) is not clear. We have identified an important role of cyclin C (CCNC) in regulating human HSPC quiescence, as knocking down CCNC expression in human cord blood CD34+ cells resulted in a significant increase in quiescent cells that maintain CD34 expression. CCNC knockdown also promotes in vitro HSPC expansion and enhances their engraftment potential in sublethally irradiated immunodeficient mice. Our studies establish cyclin C as a critical regulator of the G0/G 1 transition of human HSPCs and suggest that modulating cyclin C levels may be useful for HSC expansion and more efficient engraftment. © AlphaMed Press. |
| Keywords: | controlled study; protein expression; unclassified drug; human cell; polymerase chain reaction; ki-67 antigen; animals; mice; cells, cultured; mus; cell cycle; cell division; cell structure; rna, small interfering; cell renewal; cell differentiation; in vitro study; stem cell; blotting, western; engraftment; irradiation; hematopoietic stem cells; fibroblast; umbilical cord blood; upregulation; hematopoietic stem cell; cycline; immune deficiency; mice, inbred nod; antigens, cd34; cd34 selection; cell cycle g1 phase; cell cycle g0 phase; quiescence; cyclin c; hematopoietic stem/progenitor cell; human cord blood cd34 + cells |
| Journal Title: | Stem Cells |
| Volume: | 28 |
| Issue: | 2 |
| ISSN: | 1066-5099 |
| Publisher: | AlphaMed Press |
| Date Published: | 2010-02-01 |
| Start Page: | 308 |
| End Page: | 317 |
| Language: | English |
| DOI: | 10.1002/stem.270 |
| PUBMED: | 19967789 |
| PROVIDER: | scopus |
| PMCID: | PMC3144254 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 20 April 2011" - "CODEN: STCEE" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work