AKT is a therapeutic target in myeloproliferative neoplasms Journal Article
| Authors: | Khan, I.; Huang, Z.; Wen, Q.; Stankiewicz, M. J.; Gilles, L.; Goldenson, B.; Schultz, R.; Diebold, L.; Gurbuxani, S.; Finke, C. M.; Lasho, T. L.; Koppikar, P.; Pardanani, A.; Stein, B.; Altman, J. K.; Levine, R. L.; Tefferi, A.; Crispino, J. D. |
| Article Title: | AKT is a therapeutic target in myeloproliferative neoplasms |
| Abstract: | The majority of patients with BCR-ABL1-negative myeloproliferative neoplasms (MPN) harbor mutations in JAK2 or MPL, which lead to constitutive activation of the JAK/STAT, PI3K and ERK signaling pathways. JAK inhibitors by themselves are inadequate in producing selective clonal suppression in MPN and are associated with hematopoietic toxicities. MK-2206 is a potent allosteric AKT inhibitor that was well tolerated, including no evidence of myelosuppression, in a phase I study of solid tumors. Herein, we show that inhibition of PI3K/AKT signaling by MK-2206 affected the growth of both JAK2V617F- or MPLW515L-expressing cells via reduced phosphorylation of AKT and inhibition of its downstream signaling molecules. Moreover, we demonstrate that MK-2206 synergizes with ruxolitinib in suppressing the growth of JAK2V617F-mutant SET2 cells. Importantly, MK-2206 suppressed colony formation from hematopoietic progenitor cells in patients with primary myelofibrosis and alleviated hepatosplenomegaly and reduced megakaryocyte burden in the bone marrows, livers and spleens of mice with MPLW515L-induced MPN. Together, these findings establish AKT as a rational therapeutic target in the MPNs. © 2013 Macmillan Publishers Limited. |
| Keywords: | signal transduction; protein kinase b; controlled study; myeloproliferative disorders; myelofibrosis; human cell; mutation; janus kinase 2; histopathology; myeloid metaplasia; nonhuman; cell proliferation; mouse; animals; mice; apoptosis; enzyme inhibition; bone marrow; spleen; cell line; animal experiment; in vitro study; phosphorylation; phosphatidylinositol 3 kinase; protein kinase inhibitors; liver; neoplastic stem cells; western blotting; proto-oncogene proteins c-akt; cell cycle arrest; hematopoietic stem cell; bcr abl protein; disease models, animal; megakaryocyte; fusion proteins, bcr-abl; pi3k; myeloproliferative neoplasm; megakaryocytes; hepatosplenomegaly; colony formation; phosphatidylinositol 3-kinases; jak2; mpl; cell cycle checkpoints; 8 [4 (1 aminocyclobutyl)phenyl] 9 phenyl 1,2,4 triazolo[3,4 f][1,6]naphthyridin 3(2h) one; heterocyclic compounds, 3-ring |
| Journal Title: | Leukemia |
| Volume: | 27 |
| Issue: | 9 |
| ISSN: | 0887-6924 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2013-09-01 |
| Start Page: | 1882 |
| End Page: | 1890 |
| Language: | English |
| DOI: | 10.1038/leu.2013.167 |
| PROVIDER: | scopus |
| PUBMED: | 23748344 |
| PMCID: | PMC4023863 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 October 2013" - "CODEN: LEUKE" - "Source: Scopus" |
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