TargetScreen: An unbiased approach to identify functionally important microRNA targets Journal Article
| Authors: | Mavrakis, K. J.; Wendel, H. G. |
| Article Title: | TargetScreen: An unbiased approach to identify functionally important microRNA targets |
| Abstract: | We recently identified miR-19 as the critical activity for leukemogenesis within the oncogenic 17-92 cluster of microRNAs.1 This finding prompted us to test an unbiased method for pinpointing those miR-19 targets that may be key to its oncogenic action. Specifically, we used a large-scale short hairpin RNA screen to identify those miR-19 target genes, whose knockdown could reproduce miR-19's effects on lymphocyte transformation. In this way, we found that miR-19 produces a coordinate clampdown on multiple negative regulators of PI3K-related survival signals. These findings have implications for the therapy of miR-19 expressing tumors. They also validate a new strategy for the unbiased identification of functionally important microRNA target genes. Using the example of miR-19 in leukemia, we will discuss some possibilities and limitations of this new approach. © 2010 Landes Bioscience. |
| Keywords: | unclassified drug; review; t lymphocyte; cell survival; gene overexpression; apoptosis; microrna; gene expression; gene function; rna interference; phosphatidylinositol 3 kinase; b lymphocyte; targets; myc protein; leukemogenesis; cell cycle arrest; t cell leukemia; short hairpin rna; notch1 receptor; mir-19; t-cell leukemia; mir 19; lymphocyte transformation |
| Journal Title: | Cell Cycle |
| Volume: | 9 |
| Issue: | 11 |
| ISSN: | 1538-4101 |
| Publisher: | Taylor & Francis Inc. |
| Date Published: | 2010-06-01 |
| Start Page: | 2080 |
| End Page: | 2084 |
| Language: | English |
| DOI: | 10.4161/cc.9.11.11807 |
| PROVIDER: | scopus |
| PUBMED: | 20505335 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 20 April 2011" - "Source: Scopus" |
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