SUMO-2 and PIAS1 modulate insoluble mutant huntingtin protein accumulation Journal Article
| Authors: | O'Rourke, J. G.; Gareau, J. R.; Ochaba, J.; Song, W.; Raskó, T.; Reverter, D.; Lee, J.; Monteys, A. M.; Pallos, J.; Mee, L.; Vashishtha, M.; Apostol, B. L.; Nicholson, T. T.; Illes, K.; Zhu, Y. Z.; Dasso, M.; Bates, G. P.; DiFiglia, M.; Davidson, B.; Wanker, E. E.; Marsh, J. L.; Lima, C. D.; Steffan, J. S.; Thompson, L. M. |
| Article Title: | SUMO-2 and PIAS1 modulate insoluble mutant huntingtin protein accumulation |
| Abstract: | A key feature in Huntington disease (HD) is the accumulation of mutant Huntingtin (HTT) protein, which may be regulated by posttranslational modifications. Here, we define the primary sites of SUMOmodification in the amino-terminal domain of HTT, show modification downstream of this domain, and demonstrate that HTT is modified by the stress-inducible SUMO-2. A systematic study of E3 SUMO ligases demonstrates that PIAS1 is anE3 SUMO ligase for both HTT SUMO-1 and SUMO-2 modification and that reduction of dPIAS in a mutant HTT Drosophila model is protective. SUMO-2 modification regulates accumulation of insoluble HTT in HeLa cells in a manner that mimics proteasome inhibition and can be modulated by overexpression and acute knockdown of PIAS1. Finally, the accumulation of SUMO-2-modified proteins in the insoluble fraction of HD postmortem striata implicates SUMO-2 modification in the age-related pathogenic accumulation of mutant HTT and other cellular proteins that occurs during HD progression. © 2013 The Authors. |
| Journal Title: | Cell Reports |
| Volume: | 4 |
| Issue: | 2 |
| ISSN: | 2211-1247 |
| Publisher: | Cell Press |
| Date Published: | 2013-07-25 |
| Start Page: | 362 |
| End Page: | 375 |
| Language: | English |
| DOI: | 10.1016/j.celrep.2013.06.034 |
| PROVIDER: | scopus |
| PUBMED: | 23871671 |
| PMCID: | PMC3931302 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 4 September 2013" - "Source: Scopus" |
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