TMPRSS2-ERG rearrangement in dominant anterior prostatic tumours: Incidence and correlation with ERG immunohistochemistry Journal Article
| Authors: | Gopalan, A.; Leversha, M. A.; Dudas, M. E.; Maschino, A. C.; Chang, J.; Al-Ahmadie, H. A.; Chen, Y. B.; Tickoo, S. K.; Reuter, V. E.; Fine, S. W. |
| Article Title: | TMPRSS2-ERG rearrangement in dominant anterior prostatic tumours: Incidence and correlation with ERG immunohistochemistry |
| Abstract: | Aim: To study prostate cancer zonal differences in TMPRSS2-ERG gene rearrangement. Methods and results: We examined 136 well-characterized dominant anterior prostatic tumours, including 61 transition zone (TZ) and 75 anterior peripheral zone (PZ) lesions, defined using strict anatomical considerations. TMPRSS2-ERG FISH and ERG protein immunohistochemistry were performed on tissue microarrays. FISH results, available for 56 TZ and 71 anterior PZ samples, were correlated with ERG staining and TZ-associated 'clear cell' histology. Fewer TZ cancers (four of 56; 7%) were rearranged than anterior PZ cancers (18 of 71; 25%) (P = 0.009); deletion was the sole mechanism of TZ cancer rearrangement. ERG protein overexpression was present in 4% (two of 56; both FISH+) and 30% (21 of 71; 17 FISH+) of TZ and anterior PZ tumours, respectively. 'Clear cell' histology was present in 21 of 56 (38%) TZ and eight of 71 (11%) anterior PZ tumours. Seven per cent of cancers with and 21% without this histology had rearrangement, regardless of zonal origin. Conclusions: TMPRSS2-ERG rearrangement occurs in dominant TZ and anterior PZ prostate cancers, with all rearranged TZ cancers in this cohort showing deletion. ERG immunohistochemistry demonstrated excellent sensitivity (86%) and specificity (96%) for TMPRSS2-ERG rearrangement. TMPRSS2-ERG fusion is rare in TZ tumours and present at a low frequency in tumours displaying 'clear cell' histology. © 2013 John Wiley & Sons Ltd. |
| Keywords: | immunohistochemistry; controlled study; human tissue; protein expression; gene deletion; histopathology; cancer incidence; prostate; correlation analysis; fluorescence in situ hybridization; gene rearrangement; prostate tumor; transcription factor erg; tissue microarray; transition zone; tmprss2-erg rearrangement |
| Journal Title: | Histopathology |
| Volume: | 63 |
| Issue: | 2 |
| ISSN: | 0309-0167 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2013-08-01 |
| Start Page: | 279 |
| End Page: | 286 |
| Language: | English |
| DOI: | 10.1111/his.12153 |
| PROVIDER: | scopus |
| PMCID: | PMC3723763 |
| PUBMED: | 23701505 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 4 September 2013" - "CODEN: HISTD" - "Source: Scopus" |
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