Bioorthogonal profiling of protein methylation (BPPM) using an azido analog of S-adenosyl-L-methionine Journal Article

   


Authors: Blum, G.; Islam, K.; Luo, M.
Article Title: Bioorthogonal profiling of protein methylation (BPPM) using an azido analog of S-adenosyl-L-methionine
Abstract: Protein methyltransferases (PMTs) utilize S-adenosyl-l-methionine (SAM) as a cofactor and transfer its sulfonium methyl moiety to diverse substrates. These methylation events can lead to meaningful biological outcomes, from transcriptional activation/silencing to cell cycle regulation. This article describes recently developed technology based on protein engineering in tandem with SAM analog cofactors and bioorthogonal click chemistry to unambiguously profile the substrates of a specific PMT. The protocols encapsulate the logic and methods of selectively profiling the substrates of a candidate PMT by (1) engineering the selected PMT to accommodate a bulky SAM analog; (2) generating a proteome containing the engineered PMT; (3) visualizing the proteome-wide substrates of the designated PMT via bioorthogonal labeling with a fluorescent tag; and finally (4) pulling down the proteome-wide substrates for mass spectrometric analysis. Curr. Protoc. Chem. Biol. 5:45-66 © 2013 by John Wiley & Sons, Inc.
Keywords: click chemistry; g9a; protein engineering; synthetic cofactor
Journal Title: Current Protocols in Chemical Biology
Volume: 5
Issue: 1
ISSN: 2160-4762
Publisher: John Wiley & Sons  
Date Published: 2013-03-01
Start Page: 45
End Page: 66
Language: English
PMCID: PMC3647616
PUBMED: 23667794
DOI: 10.1002/9780470559277.ch120240
PROVIDER: manual
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MSK Authors
  1. Minkui Luo
    70 Luo
  2. Kabirul Islam
    9 Islam
  3. Gil Blum
    15 Blum
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