The trafficking protein Tmed2/p24β1 is required for morphogenesis of the mouse embryo and placenta Journal Article
| Authors: | Jerome-Majewska, L. A.; Achkar, T.; Luo, L.; Lupu, F.; Lacy, E. |
| Article Title: | The trafficking protein Tmed2/p24β1 is required for morphogenesis of the mouse embryo and placenta |
| Abstract: | During vesicular transport between the endoplasmic reticulum and the Golgi, members of the TMED/p24 protein family form hetero-oligomeric complexes that facilitate protein-cargo recognition as well as vesicle budding. In addition, they regulate each other's level of expression. Despite analyses of TMED/p24 protein distribution in mammalian cells, yeast, and C. elegans, little is known about the role of this family in vertebrate embryogenesis. We report the presence of a single point mutation in Tmed2/p24β1 in a mutant mouse line, 99J, identified in an ENU mutagenesis screen for recessive developmental abnormalities. This mutation does not affect Tmed2/p24β1 mRNA levels but results in loss of TMED2/p24β1 protein. Prior to death at mid-gestation, 99J homozygous mutant embryos exhibit developmental delay, abnormal rostral-caudal elongation, randomized heart looping, and absence of the labyrinth layer of the placenta. We find that Tmed2/p24β1 is normally expressed in tissues showing morphological defects in 99J mutant embryos and that these affected tissues lack the TMED2/p24β1 oligomerization partners, TMED7/p24β3 and TMED10/p24β1. Our data reveal a requirement for TMED2/p24β1 protein in the morphogenesis of the mouse embryo and placenta. © 2010 Elsevier Inc. |
| Keywords: | controlled study; carrier protein; unclassified drug; mutation; nonhuman; animal cell; mouse; animal; metabolism; mouse mutant; mammalia; animals; mice; animal tissue; gene expression; embryo; embryo development; morphogenesis; mice, inbred c57bl; c57bl mouse; vertebrata; animal embryo; gene expression regulation; gene expression regulation, developmental; endoplasmic reticulum; messenger rna; nucleotide sequence; homozygote; embryo, mammalian; pregnancy; caenorhabditis elegans; developmental disorder; point mutation; oligomerization; placenta; placenta development; vesicular transport proteins; abnormal heart looping; er stress; p24; posterior tail bud; tmed; vesicular transport; transmembrane emp24 domain 10 protein; transmembrane emp24 domain 2 protein; transmembrane emp24 domain 7 protein; tmed10 protein, mouse; tmed2 protein, mouse; tmed7 protein, mouse; vesicular transport protein; embryo death |
| Journal Title: | Developmental Biology |
| Volume: | 341 |
| Issue: | 1 |
| ISSN: | 0012-1606 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2010-05-01 |
| Start Page: | 154 |
| End Page: | 166 |
| Language: | English |
| DOI: | 10.1016/j.ydbio.2010.02.019 |
| PUBMED: | 20178780 |
| PROVIDER: | scopus |
| PMCID: | PMC2892723 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 20 April 2011" - "CODEN: DEBIA" - "Source: Scopus" |
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