An enzyme-linked immunosorbent assay (ELISA) for the detection of monoclonal antibodies recognizing surface antigens expressed on viable cells Journal Article


Authors: Feit, C.; Bartal, A. H.; Tauber, G.; Dymbort, G.; Hirshaut, Y.
Article Title: An enzyme-linked immunosorbent assay (ELISA) for the detection of monoclonal antibodies recognizing surface antigens expressed on viable cells
Abstract: A solid-phase enzyme-linked immunosorbent assay (ELISA) has been developed for detecting monoclonal antibodies binding to surface antigens expressed on viable adherent cells of tumor cell lines. This assay utilizes a sheep anti-mouse IgG to which a beta-galactosidase is linked. It is highly sensitive and permits quantification of IgG monoclonal antibody levels. In studies of monoclonal antibodies prepared against human tumors, the ELISA assay revealed the presence of antigens which were not seen using acetone-fixed cell immunofluorescence methods. This assay is safe, rapid, low cost, and gives reproducible quantitative results. As such it should prove useful to laboratories engaged in the study of antigens expressed on cell membranes.
Keywords: nonhuman; methodology; mouse; animals; mice; cell survival; cell line; enzyme linked immunosorbent assay; monoclonal antibody; immunoenzyme techniques; membrane antigen; antibodies, monoclonal; antigens, neoplasm; enzyme-linked immunosorbent assay; cell adhesion; antigens, surface; beta-galactosidase; ovis aries
Journal Title: Journal of Immunological Methods
Volume: 58
Issue: 3
ISSN: 0022-1759
Publisher: Elsevier Science, Inc.  
Date Published: 1983-01-01
Start Page: 301
End Page: 308
Language: English
DOI: 10.1016/0022-1759(82)90028-x
PUBMED: 6403627
PROVIDER: scopus
DOI/URL:
Notes: --- - "Export Date: 1 July 2013" - "CODEN: JIMMB" - "Source: Scopus"
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