Irreversible electroporation ablation of the liver can be detected with ultrasound B-mode and elastography Journal Article


Authors: Au, J. T.; Kingham, T. P.; Jun, K.; Haddad, D.; Gholami, S.; Mojica, K.; Monette, S.; Ezell, P.; Fong, Y.
Article Title: Irreversible electroporation ablation of the liver can be detected with ultrasound B-mode and elastography
Abstract: Background: Irreversible electroporation (IRE) is a novel ablation technique that induces permanent membrane permeability and cell death. We are interested in ultrasound B-mode and elastography to monitor IRE ablation in the liver. Methods: Yorkshire pigs underwent IRE ablation of the liver and were imaged with ultrasound B-mode and elastography. Histologic evaluation of cell death by triphenyltetrazolium chloride and hematoxylin and eosin staining was performed. Results: Elastography showed that liver ablated by IRE exhibited increased tissue stiffness with a peak strain ratio of 2.22. The IRE lesion had a discrete border without bubble artifact, and the lesion size significantly correlated with area of cell death on histology. IRE ablation was unaffected by presence of large blood vessels or bile ducts. Conclusion: IRE ablation led to increased tissue stiffness that was detectable by elastography and indicative of cell death. Elastography may complement B-mode ultrasonography to monitor IRE ablation of the liver. © 2013 Mosby, Inc. All rights reserved.
Keywords: histopathology; nonhuman; cell death; animal experiment; histology; electroporation; artifact; liver parenchyma; swine; ablation therapy; bile duct; irreversible electroporation; electric field; elastography; b scan; great blood vessel
Journal Title: Surgery
Volume: 153
Issue: 6
ISSN: 0039-6060
Publisher: Elsevier Inc.  
Date Published: 2013-06-01
Start Page: 787
End Page: 793
Language: English
DOI: 10.1016/j.surg.2012.11.022
PROVIDER: scopus
PUBMED: 23489942
DOI/URL:
Notes: --- - "Export Date: 1 July 2013" - "CODEN: SURGA" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Yuman Fong
    745 Fong
  2. T Peter Kingham
    288 Kingham
  3. Dana Haddad
    18 Haddad
  4. Sepideh Gholami
    27 Gholami
  5. Kyonghwa Jun
    3 Jun
  6. Sebastien Monette
    76 Monette
  7. Joyce Au
    12 Au
  8. Paula Cherie Ezell
    19 Ezell