Authors: | Trivedi, D.; Williams, R. Y.; O'Reilly, R. J.; Koehne, G. |
Article Title: | Generation of CMV-specific T lymphocytes using protein-spanning pools of pp65-derived overlapping pentadecapeptides for adoptive immunotherapy |
Abstract: | Cell-mediated immunity is essential for control of human cytomegalovirus (HCMV) infection. We used a pool of 138 synthetic overlapping pentadecapeptides overspanning the entire pp65 protein to generate polyclonal CMV-specific T-cell lines from 12 CMV-seropositive donors inheriting different HLA genotypes. Autologous monocyte-derived dendritic cells (DCs) pulsed with this complete pool consistently induced highly specific T cells that selectively recognized 1-3 pentadecapeptides identified by secondary responses to a mapping grid of pentadecapeptide subpools with single overlaps. Responses against peptide-loaded targets sharing single HLA class I or II alleles identified the restricting HLA alleles. HLA-A*0201(+) donors consistently responded to pentadecapeptides containing HLA-A*0201-binding epitope(aa495-503)NLVPMVATV.T-cell lines from other donors contained high frequencies of CD4 and/or CD8 T cells selectively reactive against peptides presented by other HLA alleles, including both known epitopes such (aa341-350)QYDPVAALF (HLA-A*2402) as well as unreported epitopes such as (aa267-275)HERNGFTVL (HLA-B*4001 and B*4002) and (aa513-523)FFWDANDIYRI |
Keywords: | infection; peptide; dendritic cells; cytomegalovirus; epitopes; disease; responses; allogeneic bone-marrow; marrow-transplant recipients; pp65 |
Journal Title: | Blood |
Volume: | 105 |
Issue: | 7 |
ISSN: | 0006-4971 |
Publisher: | American Society of Hematology |
Date Published: | 2005-04-01 |
Start Page: | 2793 |
End Page: | 2801 |
Language: | English |
DOI: | 10.1182/blood-2003-05-1433 |
ACCESSION: | WOS:000228042900035 |
PROVIDER: | wos |
PUBMED: | 15514011 |
Notes: | --- - Article - "Source: Wos" |