Generation of CMV-specific T lymphocytes using protein-spanning pools of pp65-derived overlapping pentadecapeptides for adoptive immunotherapy Journal Article
| Authors: | Trivedi, D.; Williams, R. Y.; O'Reilly, R. J.; Koehne, G. |
| Article Title: | Generation of CMV-specific T lymphocytes using protein-spanning pools of pp65-derived overlapping pentadecapeptides for adoptive immunotherapy |
| Abstract: | Cell-mediated immunity is essential for control of human cytomegalovirus (HCMV) infection. We used a pool of 138 synthetic overlapping pentadecapeptides overspanning the entire pp65 protein to generate polyclonal CMV-specific T-cell lines from 12 CMV-seropositive donors inheriting different HLA genotypes. Autologous monocyte-derived dendritic cells (DCs) pulsed with this complete pool consistently induced highly specific T cells that selectively recognized 1-3 pentadecapeptides identified by secondary responses to a mapping grid of pentadecapeptide subpools with single overlaps. Responses against peptide-loaded targets sharing single HLA class I or II alleles identified the restricting HLA alleles. HLA-A*0201(+) donors consistently responded to pentadecapeptides containing HLA-A*0201-binding epitope(aa495-503)NLVPMVATV.T-cell lines from other donors contained high frequencies of CD4 and/or CD8 T cells selectively reactive against peptides presented by other HLA alleles, including both known epitopes such (aa341-350)QYDPVAALF (HLA-A*2402) as well as unreported epitopes such as (aa267-275)HERNGFTVL (HLA-B*4001 and B*4002) and (aa513-523)FFWDANDIYRI |
| Keywords: | infection; peptide; dendritic cells; cytomegalovirus; epitopes; disease; responses; allogeneic bone-marrow; marrow-transplant recipients; pp65 |
| Journal Title: | Blood |
| Volume: | 105 |
| Issue: | 7 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2005-04-01 |
| Start Page: | 2793 |
| End Page: | 2801 |
| Language: | English |
| DOI: | 10.1182/blood-2003-05-1433 |
| ACCESSION: | WOS:000228042900035 |
| PROVIDER: | wos |
| PUBMED: | 15514011 |
| Notes: | --- - Article - "Source: Wos" |
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