Generation of CMV-specific T lymphocytes using protein-spanning pools of pp65-derived overlapping pentadecapeptides for adoptive immunotherapy Journal Article


Authors: Trivedi, D.; Williams, R. Y.; O'Reilly, R. J.; Koehne, G.
Article Title: Generation of CMV-specific T lymphocytes using protein-spanning pools of pp65-derived overlapping pentadecapeptides for adoptive immunotherapy
Abstract: Cell-mediated immunity is essential for control of human cytomegalovirus (HCMV) infection. We used a pool of 138 synthetic overlapping pentadecapeptides overspanning the entire pp65 protein to generate polyclonal CMV-specific T-cell lines from 12 CMV-seropositive donors inheriting different HLA genotypes. Autologous monocyte-derived dendritic cells (DCs) pulsed with this complete pool consistently induced highly specific T cells that selectively recognized 1-3 pentadecapeptides identified by secondary responses to a mapping grid of pentadecapeptide subpools with single overlaps. Responses against peptide-loaded targets sharing single HLA class I or II alleles identified the restricting HLA alleles. HLA-A*0201(+) donors consistently responded to pentadecapeptides containing HLA-A*0201-binding epitope(aa495-503)NLVPMVATV.T-cell lines from other donors contained high frequencies of CD4 and/or CD8 T cells selectively reactive against peptides presented by other HLA alleles, including both known epitopes such (aa341-350)QYDPVAALF (HLA-A*2402) as well as unreported epitopes such as (aa267-275)HERNGFTVL (HLA-B*4001 and B*4002) and (aa513-523)FFWDANDIYRI
Keywords: infection; peptide; dendritic cells; cytomegalovirus; epitopes; disease; responses; allogeneic bone-marrow; marrow-transplant recipients; pp65
Journal Title: Blood
Volume: 105
Issue: 7
ISSN: 0006-4971
Publisher: American Society of Hematology  
Date Published: 2005-04-01
Start Page: 2793
End Page: 2801
Language: English
DOI: 10.1182/blood-2003-05-1433
ACCESSION: WOS:000228042900035
PROVIDER: wos
PUBMED: 15514011
Notes: --- - Article - "Source: Wos"
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MSK Authors
  1. Deepa Trivedi
    14 Trivedi
  2. Guenther Koehne
    194 Koehne
  3. Richard O'Reilly
    701 O'Reilly