Viperin is highly induced in neutrophils and macrophages during acute and chronic lymphocytic choriomeningitis virus infection Journal Article
| Authors: | Hinson, E. R.; Joshi, N. S.; Chen, J. H.; Rahner, C.; Jung, Y. W.; Wang, X.; Kaech, S. M.; Cresswell, P. |
| Article Title: | Viperin is highly induced in neutrophils and macrophages during acute and chronic lymphocytic choriomeningitis virus infection |
| Abstract: | Although most cells are thought to respond to IFNs, there is limited information regarding specific cells that respond in vivo. Viperin is an IFN-induced antiviral protein and, therefore, is an excellent marker for IFN-responsive cells. In this study, we analyzed viperin expression in vivo during acute lymphocytic choriomeningitis virus Armstrong infection, which induces high levels of type I IFNs, and in persistently infected lymphocytic choriomeningitis virus carrier mice, which contain low levels of type I IFNs. Viperin was induced in lymphoid cells and dendritic cells (DCs) during acute infection and highly induced in neutrophils and macrophages. The expression kinetics in neutrophils, macrophages, and T and B cells paralleled IFN-α levels, but DCs expressed viperin with delayed kinetics. In carrier mice, viperin was expressed in neutrophils and macrophages but not in T and B cells or DCs. For acutely infected and carrier mice, viperin expression was IFN dependent, because treating type I IFNR knockout mice with IFN-γ- neutralizing Abs inhibited viperin expression. Viperin localized to the endoplasmic reticulum and lipid droplet-like vesicles in neutrophils. These findings delineate the kinetics and cells responding to IFNs in vivo and suggest that the profile of IFN-responsive cells changes in chronic infections. Furthermore, these data suggest that viperin may contribute to the antimicrobial activity of neutrophils. Copyright © 2010 by The American Association of Immunologists, Inc. |
| Keywords: | adolescent; protein expression; unclassified drug; genetics; interferon; nonhuman; alpha interferon; protein localization; t lymphocyte; proteins; animal cell; mouse; animal; metabolism; animals; mice; animal tissue; dendritic cell; cell protein; protein; mice, inbred c57bl; physiology; b lymphocyte; c57bl mouse; virology; chronic disease; endoplasmic reticulum; biosynthesis; immunology; neutrophil; gamma interferon; protein induction; neutrophils; macrophage; phagocytosis; macrophages; lymphoid tissue; alpha beta interferon receptor; receptor, interferon alpha-beta; acute disease; virus carrier; lymphoid cell; neutralizing antibody; fat droplet; viperin; vig1 protein, mouse; antimicrobial activity; lymphocytic choriomeningitis; lymphocytic choriomeningitis virus; interferon type i |
| Journal Title: | Journal of Immunology |
| Volume: | 184 |
| Issue: | 10 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 2010-05-15 |
| Start Page: | 5723 |
| End Page: | 5731 |
| Language: | English |
| DOI: | 10.4049/jimmunol.0903752 |
| PUBMED: | 20410488 |
| PROVIDER: | scopus |
| PMCID: | PMC3883313 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 20 April 2011" - "CODEN: JOIMA" - "Source: Scopus" |
