Pulmonary response after exposure to inhaled nickel hydroxide nanoparticles: Short and long-term studies in mice Journal Article
| Authors: | Gillespie, P. A.; Kang, G. S.; Elder, A.; Gelein, R.; Chen, L.; Moreira, A. L.; Koberstein, J.; Tchou-Wong, K. M.; Gordon, T.; Chen, L. C. |
| Article Title: | Pulmonary response after exposure to inhaled nickel hydroxide nanoparticles: Short and long-term studies in mice |
| Abstract: | Short and long-term pulmonary response to inhaled nickel hydroxide nanoparticles (nano-Ni(OH)2, CMD = 40 nm) in C57BL/6 mice was assessed using a whole body exposure system. For short-term studies mice were exposed for 4 h to nominal concentrations of 100, 500, and 1000 μg/m3. For long-term studies mice were exposed for 5 h/d, 5 d/w, for up to 5 months (m) to a nominal concentration of 100 μg/m3. Particle morphology, size distribution, chemical composition, solubility, and intrinsic oxidative capacity were determined. Markers of lung injury and inflammation in bronchoalveolar lavage fluid (BALF); histopathology; and lung tissue elemental nickel content and mRNA changes in macrophage inflammatory protein-2 (Mip-2), chemokine ligand 2 (Ccl2), interleukin 1-alpha (Il-1α), and tumor necrosis factor-alpha (Tnf-α) were assessed. Dose-related changes in BALF analyses were observed 24 h after short-term studies while significant changes were noted after 3 m and/or 5 m of exposure (24 h). Nickel content was detected in lung tissue, Ccl2 was most pronouncedly expressed, and histological changes were noted after 5 m of exposure. Collectively, data illustrates nano-Ni(OH)2 can induce inflammatory responses in C57BL/6 mice. © 2010 Informa UK Ltd. |
| Keywords: | controlled study; unclassified drug; histopathology; nonhuman; biological marker; mouse; animal tissue; mus; lung toxicity; animal experiment; inflammation; morphology; tumor necrosis factor alpha; messenger rna; nucleotide sequence; lung; nanoparticles; nanoparticle; interleukin 1alpha; oxidation; nickel; monocyte chemotactic protein 1; particle size; inhalation; solubility; lung lavage; lung parenchyma; pulmonary response; macrophage inflammatory protein 2; nickel hydroxide nanoparticle; chemical composition; exposure; lung injury |
| Journal Title: | Nanotoxicology |
| Volume: | 4 |
| Issue: | 1 |
| ISSN: | 1743-5390 |
| Publisher: | Informa Healthcare |
| Date Published: | 2010-03-01 |
| Start Page: | 106 |
| End Page: | 119 |
| Language: | English |
| DOI: | 10.3109/17435390903470101 |
| PROVIDER: | scopus |
| PMCID: | PMC2922767 |
| PUBMED: | 20730025 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 4" - "Export Date: 20 April 2011" - "Source: Scopus" |
