Oncolytic vaccinia virus therapy of salivary gland carcinoma Journal Article


Authors: Chernichenko, N.; Linkov, G.; Li, P.; Bakst, R. L.; Chen, C. H.; He, S.; Yu, Y. A.; Chen, N. G.; Szalay, A. A.; Fong, Y.; Wong, R. J.
Article Title: Oncolytic vaccinia virus therapy of salivary gland carcinoma
Abstract: Objective: To examine the therapeutic effects of an attenuated, replication-competent vaccinia virus (GLV-1h68) against a panel of 5 human salivary gland carcinoma cell lines. Design: The susceptibility of 5 salivary gland carcinoma cell lines to infection and oncolysis by GLV-1h68 was assessed in vitro and in vivo. Results: All 5 cell lines were susceptible to viral infection, transgene expression, and cytotoxic reactions. Three cell lines were exquisitely sensitive to infection by very low doses of GLV-1h68. Orthotopic parotid tumors exhibited more aggressive behavior compared with flank tumors. A single intratumoral injection of GLV-1h68 induced significant tumor regression without observed toxic effects in flank and parotid tumor models; controls demonstrated rapid tumor progression. Conclusion: These promising results demonstrate significant oncolytic activity by an attenuated vaccinia virus for infecting and lysing salivary gland carcinomas, supporting future clinical trials. © 2013 American Medical Association. All rights reserved.
Keywords: unclassified drug; human cell; nonhuman; mouse; animal tissue; gene expression; green fluorescent protein; luciferase; animal experiment; in vivo study; cancer cell culture; cytotoxicity; in vitro study; enzyme activity; cancer regression; human cell culture; xenograft; cancer size; beta galactosidase; oncolytic virus; oncolytic virotherapy; vaccinia virus; glv 1h68; virus replication; salivary gland carcinoma; infection sensitivity
Journal Title: JAMA Otolaryngology - Head and Neck Surgery
Volume: 139
Issue: 2
ISSN: 2168-6181
Publisher: American Medical Association  
Date Published: 2013-02-01
Start Page: 173
End Page: 182
Language: English
PROVIDER: scopus
PUBMED: 23429949
DOI: 10.1001/jamaoto.2013.1360
PMCID: PMC4413455
DOI/URL:
Notes: --- - "Export Date: 1 May 2013" - ":doi 10.1001/jamaoto.2013.1360" - "Source: Scopus"
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MSK Authors
  1. Richard J Wong
    230 Wong
  2. Richard L Bakst
    15 Bakst
  3. Yuman Fong
    745 Fong
  4. Chun-Hao Chen
    35 Chen
  5. Shuangba He
    11 He
  6. Pingdong Li
    5 Li
  7. Gary Linkov
    6 Linkov