Effects of VEGF on adhesion of mammary carcinoma cells to brain microvascular endothelium Conference Paper
| Authors: | Fan, J.; Cai, B.; Hao, Y.; Giancotti, F. G.; Fu, B. M. |
| Title: | Effects of VEGF on adhesion of mammary carcinoma cells to brain microvascular endothelium |
| Conference Title: | 2010 IEEE 36th Annual Northeast Bioengineering Conference (NEBEC 2010) |
| Abstract: | Vascular endothelial growth factor (VEGF) has been shown to increase the adhesion of human breast cancer cells to human brain endothelium [1]. However, how VEGF accomplishes this is not well understood. We hypothesize that VEGF compromises the integrity of brain microvascular endothelium is one mechanism to increase the tumor cell adhesion. To test our hypothesis, using a Transwell system, we measured the permeability of a cell monolayer of bEnd3 to FITC-labeled BSA, PBSA. We observed that PBSA significantly increased from 4.17±0.67×10-7cm/s to 5.20±0. 31×10-7cm/s after 5-60 min treatment with 1 nM VEGF. Correspondingly, after 60 min treatment with 1 nM VEGF, the adherent mouse ErB2-transformed mammary carcinoma cells increased from 38±13/mm 2 to 67±8/mm2 for a wild type; from 40±5/mm2 to 56±3/mm2 for the β4 mutant. Pretreatment of the cancer cells with α6, β1, α5 integrin antibodies and pretreatment of the cell monolayer with laminin-5 antibody significantly decreased the adhesion of WT and 1355T. Our results suggest that α6β4, α6β1, α5β1 and their ligand, laminin-5, play a role in breast tumor cell adhesion, and that VEGF enhances the adhesion by compromising the integrity of bEnd3 monolayer. ©2010 IEEE. |
| Keywords: | breast cancer; endothelial cells; tumors; vascular endothelial growth factor; wild types; body fluids; antibodies; cell adhesion; cancer cells; laminin; integrins; vegf; human brain; adhesion; permeability; pre-treatment; endothelial; breast tumor cells; cell monolayers; endothelial permeability; human breast cancer cells; mammary carcinoma cell; microvascular endothelium; tumor cell adhesion; capillarity; monolayers; self assembly |
| Journal Title | Annual Northeast Biomedical Engineering Conference. Proceedings |
| Conference Dates: | 2010 Mar 26-28 |
| Conference Location: | New York, NY |
| ISBN: | 1071-121X |
| Publisher: | IEEE |
| Location: | New York, NY |
| Date Published: | 2010-01-01 |
| Language: | English |
| DOI: | 10.1109/NEBC.2010.5458244 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010 - Proc. IEEE Annu. Northeast Bioeng. Conf., NEBEC - March 26-28, 2010 - New York, NY - "Conference code: 80511" - "Article No: 5458244" - "Source: Scopus" - "Export Date: 20 April 2011" |
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