Oestrogenic regulation and differential expression of WNT4 in the bonnet monkey and rodent epididymis Journal Article
| Authors: | Vijayakumar, G.; Deshpande, S. N.; Rao, A. J. |
| Article Title: | Oestrogenic regulation and differential expression of WNT4 in the bonnet monkey and rodent epididymis |
| Abstract: | A role for oestrogen in regulating fluid reabsorption in the monkey epididymis was recently demonstrated. Here, these studies are extended to identify potential oestrogen-regulated proteins in the cauda region of monkey epididymis treated with vehicle and oestrogen receptor antagonist (ICI 182780). Two-dimensional electrophoretic analysis was used to identify the proteins. The results indicated down-regulation of WNT4 in the ICI-182780-treated monkey cauda. In addition, the Wnt4 mRNA concentration was also reduced in the caput regions of ICI-182780-treated rats and oestrogen receptor knockout mice. WNT4 is a key regulator of gonadal differentiation in humans and mice and plays a pivotal role in early mouse embryogenesis. The results of the present study establish the presence of WNT4 in the monkey epididymis and its regulation by oestrogen, and suggest a role for WNT4 in maintaining epididymal homeostasis. © 2009 Published by Reproductive Healthcare Ltd. |
| Keywords: | controlled study; protein expression; nonhuman; protein function; mass spectrometry; protein analysis; animal cell; animals; mice; mice, knockout; gene expression; estrogen; estrogens; embryo development; leydig cell; amino acid sequence; messenger rna; reverse transcriptase polymerase chain reaction; rat; down regulation; receptors, estrogen; rats; dna primers; estrogen receptor; sequence homology; homeostasis; epididymis; monkey; oestrogen; wnt4; rna 16s; wnt4 protein; epididymis tail; experimental monkey; gonad development; hormonal regulation; matrix assisted laser desorption ionization time of flight mass spectrometry; two dimensional electrophoresis; electrophoresis, gel, two-dimensional; estradiol; macaca radiata; rats, wistar; water-electrolyte balance; wnt proteins |
| Journal Title: | Reproductive BioMedicine Online |
| Volume: | 18 |
| Issue: | 4 |
| ISSN: | 1472-6483 |
| Publisher: | Reproductive Healthcare Ltd |
| Date Published: | 2009-04-01 |
| Start Page: | 555 |
| End Page: | 561 |
| Language: | English |
| PUBMED: | 19400999 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 30 November 2010" - "CODEN: RBOEA" - "Source: Scopus" |
