GSK3β affects apical-basal polarity and cell-cell adhesion by regulating aPKC levels Journal Article
| Authors: | Colosimo, P. F.; Liu, X.; Kaplan, N. A.; Tolwinski, N. S. |
| Article Title: | GSK3β affects apical-basal polarity and cell-cell adhesion by regulating aPKC levels |
| Abstract: | The dynamic rearrangement of cell-cell contacts is required for the establishment of functional epithelial cell sheets. However, the signaling pathways and cellular mechanisms that initiate and maintain this polarity are not well understood. We show that loss of the Wnt signaling component GSK3β results in increased levels of aPKC and leads to defects in apical-basal polarity. We find that GSK3β directly phosphorylates aPKC, which likely promotes its ubiquitin-mediated proteosomal degradation. aPKC increases the levels of Armadillo and stabilizes adherens junctions. These results suggest that the Wnt pathway component GSK3β regulates the polarity determinant aPKC, which in turn affects cell-cell contacts during the development of polarized tissues. © 2009 Wiley-Liss, Inc. |
| Keywords: | signal transduction; controlled study; protein phosphorylation; human cell; genetics; nonhuman; ubiquitin; animal; cytology; metabolism; animals; proteasome; basement membrane; embryo; protein degradation; drosophila; enzyme activity; physiology; gene expression regulation; gene expression regulation, developmental; blotting, western; fluorescent antibody technique; protein processing; ubiquitination; glycogen synthase kinase 3beta; western blotting; immunoprecipitation; epithelium cell; epithelial cells; cell polarity; protein kinase c; cell junction; wnt proteins; cell adhesion; cell communication; wnt protein; polarization; armadillo domain protein; apical membrane; armadillo; tissue; organogenesis; polarity; wnt; glycogen synthase kinase 3; adherens junctions; β-catenin; atypical pkc; glycogen synthase kinase 3 beta; pkc 3 protein; pkc-3 protein; cross breeding; crosses, genetic |
| Journal Title: | Developmental Dynamics |
| Volume: | 239 |
| Issue: | 1 |
| ISSN: | 1058-8388 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 2010-01-01 |
| Start Page: | 115 |
| End Page: | 125 |
| Language: | English |
| DOI: | 10.1002/dvdy.21963 |
| PUBMED: | 19422025 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 20 April 2011" - "CODEN: DEDYE" - "Source: Scopus" |
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