Drug combination studies and their synergy quantification using the chou-talalay method Journal Article
| Author: | Chou, T. C. |
| Article Title: | Drug combination studies and their synergy quantification using the chou-talalay method |
| Abstract: | This brief perspective article focuses on the most common errors and pitfalls, as well as the do's and don'ts in drug combination studies, in terms of experimental design, data acquisition, data interpretation, and computerized simulation. The Chou-Talalay method for drug combination is based on the median-effect equation, derived from the mass-action law principle, which is the unified theory that provides the common link between single entity and multiple entities, and first order and higher order dynamics. This general equation encompasses the Michaelis-Menten, Hill, Henderson-Hasselbalch, and Scatchard equations in biochemistry and biophysics. The resulting combination index (CI) theorem of Chou-Talalay offers quantitative definition for additive effect (CI = 1), synergism (CI < 1), and antagonism (CI > 1) in drug combinations. This theory also provides algorithms for automated computer simulation for synergism and/or antagonism at any effect and dose level, as shown in the CI plot and isobologram, respectively. ©2010 AACR. |
| Keywords: | clinical trial; review; cisplatin; drug potentiation; nonhuman; alpha interferon; paclitaxel; topotecan; human immunodeficiency virus infection; animals; algorithms; prediction; drug synergism; drug antagonism; statistical analysis; drug therapy, combination; algorithm; virus rna; acetylsalicylic acid; computer simulation; acquired immune deficiency syndrome; drug dose regimen; biochemistry; ribavirin; zidovudine; lamivudine; chou talalay method; biophysics; michaelis constant; pharmaceutical preparations; henderson nursing model; mass action; scatchard plot |
| Journal Title: | Cancer Research |
| Volume: | 70 |
| Issue: | 2 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2010-01-15 |
| Start Page: | 440 |
| End Page: | 446 |
| Language: | English |
| DOI: | 10.1158/0008-5472.can-09-1947 |
| PUBMED: | 20068163 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 25" - "Export Date: 20 April 2011" - "CODEN: CNREA" - "Source: Scopus" |
