Imaging of tumor vascularization using fluorescence molecular tomography to monitor arginine deiminase treatment in melanoma Journal Article
| Authors: | Stelter, L.; Evans, M. J.; Jungbluth, A. A.; Longo, V. A.; Zanzonico, P.; Ritter, G.; Bomalaski, J. S.; Old, L.; Larson, S. M. |
| Article Title: | Imaging of tumor vascularization using fluorescence molecular tomography to monitor arginine deiminase treatment in melanoma |
| Abstract: | Based on their inability to express argininosuccinate synthetase (ASS), some cancer entities feature the characteristic of L-arginine (Arg) auxotrophy. This inability to intrinsically generate Argmakes them applicable for arginine deiminase (ADI) treatment, an Arg-depleting drug. Arg is also used for the synthesis of endothelial nitric oxide (NO), which mainly confers vasodilatation but is also considered to have amajor influence on tumor vascularization. The purpose of this study was to define changes in tumor vasculature in an ADI-treated melanoma xenograft mouse model using the blood pool agent AngioSense 750 and fluorescence molecular tomography (FMT). We used an ASSnegative melanomaxenograftmouse model and subjected it toweekly ADI treatment. Changes in tumor size weremeasured, and alterations in tumor vasculature were depicted by FMT and CD31 immunohistochemistry (IHC). On ADI treatment and effective antitumor therapy, we observed a drop in NO plasma levels and visualized changes in tumor vascularization with FMT and IHC. ADI treatment in melanoma xenografts has a tumor-reducing effect, which can be noninvasively imaged by quantifying tumor vascularization with FMT and IHC. © 2013 Decker Publishing. |
| Journal Title: | Molecular Imaging |
| Volume: | 12 |
| Issue: | 1 |
| ISSN: | 1535-3508 |
| Publisher: | Sage Publications, Inc. |
| Date Published: | 2013-02-01 |
| Start Page: | 67 |
| End Page: | 73 |
| Language: | English |
| DOI: | 10.2310/7290.2012.00009 |
| PROVIDER: | scopus |
| PUBMED: | 23348793 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 March 2013" - "Source: Scopus" |
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