Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and-independent autophagy Journal Article


Authors: Gammoh, N.; Florey, O.; Overholtzer, M.; Jiang, X.
Article Title: Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and-independent autophagy
Abstract: Autophagy is a finely orchestrated cellular catabolic process that requires multiple autophagy-related gene products (ATG proteins). The ULK1 complex functions to integrate upstream signals to downstream ATG proteins through an unknown mechanism. Here we have identified an interaction between mammalian FIP200 and ATG16L1, essential components of the ULK1 and ATG5 complexes, respectively. Further analyses show this is a direct interaction mediated by a short domain of ATG16L1 that we term the FIP200-binding domain (FBD). The FBD is not required for ATG16L1 self-dimerization or interaction with ATG5. Notably, an FBD-deleted ATG16L1 mutant is defective in mediating amino acid starvation-induced autophagy, which requires the ULK1 complex. However, this mutant retains its function in supporting glucose deprivation-induced autophagy, a ULK1 complex-independent process. This study therefore identifies a previously uncharacterized interaction between the ULK1 and ATG5 complexes that can distinguish ULK1-dependent and-independent autophagy processes. © 2013 Nature America, Inc. All rights reserved.
Journal Title: Nature Structural and Molecular Biology
Volume: 20
Issue: 2
ISSN: 1545-9993
Publisher: Nature Publishing Group  
Date Published: 2013-02-01
Start Page: 144
End Page: 149
Language: English
DOI: 10.1038/nsmb.2475
PROVIDER: scopus
PMCID: PMC3565010
PUBMED: 23262492
DOI/URL:
Notes: --- - "Export Date: 1 March 2013" - "CODEN: NSMBC" - "Source: Scopus"
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  1. Noor I Gammoh
    8 Gammoh
  2. Oliver Florey
    10 Florey
  3. Xuejun Jiang
    124 Jiang