Cancer-testis antigen expression and immunogenicity in AL amyloidosis Journal Article

Authors: Rosenzweig, M. A.; Landau, H.; Seldin, D.; Ohara, C.; Girnius, S.; Hanson, N.; Frosina, D.; Sedrak, C.; Arcila, M.; Comenzo, R. L.; Giralt, S.; Gnjatic, S.; Jungbluth, A. A.; Koehne, G.
Article Title: Cancer-testis antigen expression and immunogenicity in AL amyloidosis
Abstract: Light-chain amyloidosis (AL) is a plasma cell dyscrasia closely related to multiple myeloma. In multiple myeloma, the cancer-testis antigens (CTAs) CT7 (MAGE-C1), CT10 (MAGE-C2) and MAGE-A CTAs are expressed in up to 80% of cases. In this study, we investigated the expression and immunogenicity of several CTAs in patients with AL amyloidosis in a total of 38 bone marrow specimens by employing standard immunohistochemistry techniques on paraffin-embedded archival tissues. Plasma samples from 35 patients (27 with matched bone marrow samples) were also analyzed by ELISA for sero reactivity to a group of full-length CTA proteins. CT7 was present in 25/38 (66%) while CT10 was demonstrated in 3/38 and GAGE in 1/38 AL amyloid cases. The expression pattern was mostly focal. There were no significant differences with regard to organ involvement, response to treatment, or prognosis in CTA positive compared to negative cases. None of the specimens showed spontaneous humoral immunity to CT7, but sero reactivity was observed in individual patients to other CTAs. This study identifies CT7 as the prevalent CTA in plasma cells of patients with AL amyloidosis. Further analyses determining the biology of CTAs in AL amyloidosis and their value as potential targets for immunotherapy are warranted. © 2012 Macmillan Publishers Limited All rights reserved.
Keywords: immunohistochemistry; adult; clinical article; human tissue; treatment response; drug targeting; antigen expression; cancer immunotherapy; amyloidosis; stem cell transplantation; immunoreactivity; enzyme linked immunosorbent assay; tissue section; plasma cell; tissue distribution; bone marrow biopsy; cancer testis antigen; antigen detection; immunogenicity; light chain amyloidosis; humoral immunity; cancer-testis antigens; plasma cell dyscrasia; al amyloidosis
Journal Title: Blood Cancer Journal
Volume: 2
Issue: 9
ISSN: 2044-5385
Publisher: Nature Publishing Group  
Date Published: 2012-09-14
Start Page: e90
Language: English
DOI: 10.1038/bcj.2012.32
PROVIDER: scopus
PMCID: PMC3461704
PUBMED: 22983433
Notes: --- - "Export Date: 14 February 2013" - "Source: Scopus"
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MSK Authors
  1. Sergio Andres Giralt
    537 Giralt
  2. Guenther Koehne
    175 Koehne
  3. Heather Jolie Landau
    189 Landau
  4. Sacha Gnjatic
    111 Gnjatic
  5. Achim Jungbluth
    341 Jungbluth
  6. Maria Eugenia Arcila
    350 Arcila
  7. Denise Frosina
    84 Frosina
  8. Nicole C Hanson
    11 Hanson
  9. Christine Sedrak
    4 Sedrak