Verification and unmasking of widely used human esophageal adenocarcinoma cell lines Journal Article
| Authors: | Boonstra, J. J.; Van Marion, R.; Beer, D. G.; Lin, L.; Chaves, P.; Ribeiro, C.; Pereira, A. D.; Roque, L.; Darnton, S. J.; Altorki, N. K.; Schrump, D. S.; Klimstra, D. S.; Tang, L. H.; Eshleman, J. R.; Alvarez, H.; Shimada, Y.; Van Dekken, H.; Tilanus, H. W.; Dinjens, W. N. M. |
| Article Title: | Verification and unmasking of widely used human esophageal adenocarcinoma cell lines |
| Abstract: | For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the cell line. In a worldwide effort, we verified the authenticity of all available esophageal adenocarcinoma (EAC) cell lines. We proved that the frequently used cell lines SEG-1 and BIC-1 and the SK-GT-5 cell line are in fact cell lines from other tumor types. Experimental results based on these contaminated cell lines have led to ongoing clinical trials recruiting EAC patients, to more than 100 scientific publications, and to at least three National Institutes of Health cancer research grants and 11 US patents, which emphasizes the importance of our findings. Widespread use of contaminated cell lines threatens the development of treatment strategies for EAC. |
| Keywords: | mitogen activated protein kinase; unclassified drug; gene mutation; gene sequence; clinical trial; sorafenib; raf protein; antineoplastic agents; benzenesulfonates; clinical trials as topic; pyridines; united states; adenocarcinoma; cell proliferation; apoptosis; biomedical research; lung neoplasms; genotype; antineoplastic activity; cancer cell culture; cell line, tumor; protein p53; telomerase; cancer research; carcinogenesis; colorectal carcinoma; protein kinase inhibitors; colorectal neoplasms; nucleotide sequence; carcinoma; dna fingerprinting; esophageal adenocarcinoma; oligopeptides; stomach neoplasms; esophageal neoplasms; national institutes of health (u.s.); grn 163l; telomerase inhibitor; short tandem repeat; carcinoma, large cell; tandem repeat sequences |
| Journal Title: | JNCI: Journal of the National Cancer Institute |
| Volume: | 102 |
| Issue: | 4 |
| ISSN: | 0027-8874 |
| Publisher: | Oxford University Press |
| Date Published: | 2010-02-24 |
| Start Page: | 271 |
| End Page: | 274 |
| Language: | English |
| DOI: | 10.1093/jnci/djp499 |
| PUBMED: | 20075370 |
| PROVIDER: | scopus |
| PMCID: | PMC2902814 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 12" - "Export Date: 20 April 2011" - "CODEN: JNCIA" - "Source: Scopus" |
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