Genome-wide identification of miRNA targets by PAR-CLIP Journal Article


Authors: Hafner, M.; Lianoglou, S.; Tuschl, T.; Betel, D.
Article Title: Genome-wide identification of miRNA targets by PAR-CLIP
Abstract: miRNAs are short (20-23 nt) RNAs that are loaded into proteins of the Argonaute (AGO) family and guide them to partially complementary target sites on mRNAs, resulting in mRNA destabilization and/or translational repression. It is estimated that about 60% of the mammalian genes are potentially regulated by miRNAs, and therefore methods for experimental miRNA target determination have become valuable tools for the characterization of posttranscriptional gene regulation. Here we present a step-by-step protocol and guidelines for the computational analysis for the large-scale identification of miRNA target sites in cultured cells by photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (PAR-CLIP) of AGO proteins. © 2012 Elsevier Inc.
Keywords: controlled study; unclassified drug; human cell; mammalia; microrna; genetic association; cell culture; gene identification; immunoprecipitation; binding site; bioinformatics; argonaute protein; photoactivation; mirna; microrna 302; microrna 20a; clip; next-generation sequencing; microrna 130; microrna 144; microrna 19b 1; microrna 301; microrna 372; microrna 373; microrna 454; microrna 520; microrna 92a 1; photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation
Journal Title: Methods
Volume: 58
Issue: 2
ISSN: 1046-2023
Publisher: Academic Press Inc., Elsevier Science  
Date Published: 2012-10-01
Start Page: 94
End Page: 105
Language: English
DOI: 10.1016/j.ymeth.2012.08.006
PROVIDER: scopus
PMCID: PMC3508682
PUBMED: 22926237
DOI/URL:
Notes: --- - "Cited By (since 1996): 1" - "Export Date: 2 January 2013" - "CODEN: MTHDE" - "Source: Scopus"
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