Genome-wide identification of miRNA targets by PAR-CLIP Journal Article
| Authors: | Hafner, M.; Lianoglou, S.; Tuschl, T.; Betel, D. |
| Article Title: | Genome-wide identification of miRNA targets by PAR-CLIP |
| Abstract: | miRNAs are short (20-23 nt) RNAs that are loaded into proteins of the Argonaute (AGO) family and guide them to partially complementary target sites on mRNAs, resulting in mRNA destabilization and/or translational repression. It is estimated that about 60% of the mammalian genes are potentially regulated by miRNAs, and therefore methods for experimental miRNA target determination have become valuable tools for the characterization of posttranscriptional gene regulation. Here we present a step-by-step protocol and guidelines for the computational analysis for the large-scale identification of miRNA target sites in cultured cells by photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (PAR-CLIP) of AGO proteins. © 2012 Elsevier Inc. |
| Keywords: | controlled study; unclassified drug; human cell; mammalia; microrna; genetic association; cell culture; gene identification; immunoprecipitation; binding site; bioinformatics; argonaute protein; photoactivation; mirna; microrna 302; microrna 20a; clip; next-generation sequencing; microrna 130; microrna 144; microrna 19b 1; microrna 301; microrna 372; microrna 373; microrna 454; microrna 520; microrna 92a 1; photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation |
| Journal Title: | Methods |
| Volume: | 58 |
| Issue: | 2 |
| ISSN: | 1046-2023 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 2012-10-01 |
| Start Page: | 94 |
| End Page: | 105 |
| Language: | English |
| DOI: | 10.1016/j.ymeth.2012.08.006 |
| PROVIDER: | scopus |
| PMCID: | PMC3508682 |
| PUBMED: | 22926237 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 2 January 2013" - "CODEN: MTHDE" - "Source: Scopus" |
