Transcription factor Foxp3 and its protein partners form a complex regulatory network Journal Article


Authors: Rudra, D.; Deroos, P.; Chaudhry, A.; Niec, R. E.; Arvey, A.; Samstein, R. M.; Leslie, C.; Shaffer, S. A.; Goodlett, D. R.; Rudensky, A. Y.
Article Title: Transcription factor Foxp3 and its protein partners form a complex regulatory network
Abstract: The transcription factor Foxp3 is indispensible for the differentiation and function of regulatory T cells (T reg cells). To gain insights into the molecular mechanisms of Foxp3-mediated gene expression, we purified Foxp3 complexes and explored their composition. Biochemical and mass-spectrometric analyses revealed that Foxp3 forms multiprotein complexes of 400-800 kDa or larger and identified 361 associated proteins, ∼30% of which were transcription related. Foxp3 directly regulated expression of a large proportion of the genes encoding its cofactors. Some transcription factor partners of Foxp3 facilitated its expression. Functional analysis of the cooperation of Foxp3 with one such partner, GATA-3, provided additional evidence for a network of transcriptional regulation afforded by Foxp3 and its associates to control distinct aspects of T reg cell biology. © 2012 Nature America, Inc. All rights reserved.
Keywords: controlled study; unclassified drug; nonhuman; transcription factor foxp3; forkhead transcription factors; mass spectrometry; animal cell; mouse; animals; mice; complex formation; transcription factor gata 3; protein protein interaction; transcription factor; cell differentiation; proteomics; mice, transgenic; gene expression regulation; cd4+ cd25+ t lymphocyte; regulatory t lymphocyte; transcription regulation; t-lymphocytes, regulatory; nucleotide sequence; protein structure, tertiary; biotinylation; gene regulatory networks; transcription factor foxp1; transcription factor ash21; transcription factor bcl 11b; transcription factor cbfbeta; transcription factor chd4; transcription factor hdac2; transcription factor lkzf3; transcription factor lsd1; transcription factor rcor1; transcription factor smarca4; transcription factor smarca5; transcription factor smarcc1; transcription factor smarce1; gata3 transcription factor
Journal Title: Nature Immunology
Volume: 13
Issue: 10
ISSN: 1529-2908
Publisher: Nature Publishing Group  
Date Published: 2012-10-01
Start Page: 1010
End Page: 1019
Language: English
DOI: 10.1038/ni.2402
PROVIDER: scopus
PMCID: PMC3448012
PUBMED: 22922362
DOI/URL:
Notes: --- - "Cited By (since 1996): 2" - "Export Date: 2 November 2012" - "CODEN: NIAMC" - "Molecular Sequence Numbers: GENBANK: GSE39864;" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Alexander Rudensky
    100 Rudensky
  2. Dipayan Rudra
    4 Rudra
  3. Paul C Deroos
    2 Deroos
  4. Christina Leslie
    111 Leslie
  5. Aaron J. Arvey
    20 Arvey
  6. Rachel E Niec
    8 Niec