Transcription factor Foxp3 and its protein partners form a complex regulatory network Journal Article
| Authors: | Rudra, D.; Deroos, P.; Chaudhry, A.; Niec, R. E.; Arvey, A.; Samstein, R. M.; Leslie, C.; Shaffer, S. A.; Goodlett, D. R.; Rudensky, A. Y. |
| Article Title: | Transcription factor Foxp3 and its protein partners form a complex regulatory network |
| Abstract: | The transcription factor Foxp3 is indispensible for the differentiation and function of regulatory T cells (T reg cells). To gain insights into the molecular mechanisms of Foxp3-mediated gene expression, we purified Foxp3 complexes and explored their composition. Biochemical and mass-spectrometric analyses revealed that Foxp3 forms multiprotein complexes of 400-800 kDa or larger and identified 361 associated proteins, ∼30% of which were transcription related. Foxp3 directly regulated expression of a large proportion of the genes encoding its cofactors. Some transcription factor partners of Foxp3 facilitated its expression. Functional analysis of the cooperation of Foxp3 with one such partner, GATA-3, provided additional evidence for a network of transcriptional regulation afforded by Foxp3 and its associates to control distinct aspects of T reg cell biology. © 2012 Nature America, Inc. All rights reserved. |
| Keywords: | controlled study; unclassified drug; nonhuman; transcription factor foxp3; forkhead transcription factors; mass spectrometry; animal cell; mouse; animals; mice; complex formation; transcription factor gata 3; protein protein interaction; transcription factor; cell differentiation; proteomics; mice, transgenic; gene expression regulation; cd4+ cd25+ t lymphocyte; regulatory t lymphocyte; transcription regulation; t-lymphocytes, regulatory; nucleotide sequence; protein structure, tertiary; biotinylation; gene regulatory networks; transcription factor foxp1; transcription factor ash21; transcription factor bcl 11b; transcription factor cbfbeta; transcription factor chd4; transcription factor hdac2; transcription factor lkzf3; transcription factor lsd1; transcription factor rcor1; transcription factor smarca4; transcription factor smarca5; transcription factor smarcc1; transcription factor smarce1; gata3 transcription factor |
| Journal Title: | Nature Immunology |
| Volume: | 13 |
| Issue: | 10 |
| ISSN: | 1529-2908 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2012-10-01 |
| Start Page: | 1010 |
| End Page: | 1019 |
| Language: | English |
| DOI: | 10.1038/ni.2402 |
| PROVIDER: | scopus |
| PMCID: | PMC3448012 |
| PUBMED: | 22922362 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 2 November 2012" - "CODEN: NIAMC" - "Molecular Sequence Numbers: GENBANK: GSE39864;" - "Source: Scopus" |
