Treatment and eradication of murine fur mites: II. Diagnostic considerations Journal Article
| Authors: | Arbona, R. J. ; Lipman, N. S.; Wolf, F. R. |
| Article Title: | Treatment and eradication of murine fur mites: II. Diagnostic considerations |
| Abstract: | Fur mites are a persistent problem in contemporary laboratory mouse colonies. We conducted several studies to evaluate fur mite diagnostic methodologies and interpretation of results. Retrospective analysis of test results from sentinel mice exposed to soiled bedding collected from colonies infested with Myobia musculi and Myocoptes musculinus revealed the skin scrape test to be more reliable than pelt examination, provided that both the head and dorsal thoracolumbar regions were sampled. To assess their diagnostic accuracy, 3 commercial laboratories were sent positive control slides containing mites, mite parts, or eggs in sets of slides containing diagnostic skin scrapings in varying ratios. Laboratory B correctly identified the positive control slide. Laboratory A identified 1 of 3 positive control slides, whereas laboratory C failed to identify both positive control slides submitted. To determine the time required for a mouse to shed its entire hair coat, fur of Crl:CD1(ICR), BALB/cAnNCrl, and Crl:CFW(SW) albino mice was dyed black and the presence of dyed fur evaluated monthly for 8 mo. Limited dyed hair was still present at 8 mo; therefore, finding eggs or egg casings many months after treatment cessation does not necessarily imply treatment failure. To evaluate the effectiveness of soiled bedding sentinels for detection of fur mites in a mite-infested colony, we exposed naïve mice to varying amounts (100%, 50%, 25%, 2.5%, and 0%) of soiled bedding in clean bedding. As little as 2.5% soiled bedding resulted in detection of a positive sentinel within a 2-mo period. Copyright 2010 by the American Association for Laboratory Animal Science. |
| Keywords: | controlled study; treatment failure; nonhuman; diagnostic accuracy; mouse; mus; animal experiment; animal model; retrospective study; experimental animal; diagnostic value; murinae; reliability; diagnostic test; mite; mite infestation; myobia musculi; myocoptes musculinus; acari; myocoptes |
| Journal Title: | Journal of the American Association for Laboratory Animal Science |
| Volume: | 49 |
| Issue: | 5 |
| ISSN: | 1559-6109 |
| Publisher: | American Association for Laboratory Animal Science |
| Date Published: | 2010-09-01 |
| Start Page: | 583 |
| End Page: | 587 |
| Language: | English |
| PROVIDER: | scopus |
| PMCID: | PMC2949427 |
| PUBMED: | 20858359 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 20 April 2011" - "Source: Scopus" |
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