Performance characteristics of the Cepheid Xpert vanA assay for rapid identification of patients at high risk for carriage of vancomycin-resistant enterococci Journal Article

   


Authors: Babady, N. E.; Gilhuley, K.; Cianciminio Bordelon, D.; Tang, Y. W.
Article Title: Performance characteristics of the Cepheid Xpert vanA assay for rapid identification of patients at high risk for carriage of vancomycin-resistant enterococci
Abstract: We compared the performance characteristics of culture and the Cepheid Xpert vanA assay for routine surveillance of vancomycin-resistant enterococci (VRE) from rectal swabs in patients at high risk for VRE carriage. The Cepheid Xpert vanA assay had a limit of detection of 100 CFU/ml and correctly detected 101 well-characterized clinical VRE isolates with no cross-reactivity in 27 non-VRE and related culture isolates. The clinical sensitivity, specificity, positive predictive value, and negative predictive value of the Xpert vanA PCR assay were 100%, 96.9%, 91.3%, and 100%, respectively, when tested on 300 consecutively collected rectal swabs. This assay provides excellent predictive values for prompt identification of VRE-colonized patients in hospitals with relatively high rates of VRE carriage. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
Keywords: controlled study; nonhuman; sensitivity and specificity; assay; prediction; intermethod comparison; bacterium isolate; high risk population; bacterial colonization; vancomycin resistant enterococcus; vancomycin; cross reaction; bacterium culture; bacterium identification; microbiological examination; infection risk; reference value; colony forming unit; disease surveillance; diagnostic test accuracy study; teicoplanin; minimum inhibitory concentration; bacterium carrier; amphotericin b; cefoperazone; rectal swab
Journal Title: Journal of Clinical Microbiology
Volume: 50
Issue: 11
ISSN: 0095-1137
Publisher: American Society for Microbiology  
Date Published: 2012-11-01
Start Page: 3659
End Page: 3663
Language: English
DOI: 10.1128/jcm.01776-12
PROVIDER: scopus
PUBMED: 22972822
PMCID: PMC3486258
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84867497236&partnerID=40&md5=4f10cf4077a79bcd06c9c52de1fd3f58
Notes: --- - "Export Date: 2 November 2012" - "CODEN: JCMID" - "Source: Scopus"
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MSK Authors
  1. Ngolela Esther Babady
    171 Babady
  2. Kathleen A Gilhuley
    12 Gilhuley
  3. Yi-Wei Tang
    188 Tang
  4. Diane Cianciminio-Bordelon
    2 Cianciminio-Bordelon
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