Gene expression profiles of serous, endometrioid, and clear cell subtypes of ovarian and endometrial cancer Journal Article
| Authors: | Zorn, K. K.; Bonome, T.; Gangi, L.; Chandramouli, G. V. R.; Awtrey, C. S.; Gardner, G. J.; Barrett, J. C.; Boyd, J.; Birrer, M. J. |
| Article Title: | Gene expression profiles of serous, endometrioid, and clear cell subtypes of ovarian and endometrial cancer |
| Abstract: | Purpose: The presence of similar histologic subtypes of epithelial ovarian and endometrial cancers has long been noted, although the relevance of this finding to pathogenesis and clinical management is unclear. Despite similar clinical characteristics, histologic subtypes of cancers of the ovary and endometrium are treated according to organ of origin. This study compares the gene expression profiles of analogous histologic subtypes of cancers of the ovary and endometrium using the same genomic platform to determine the similarities and differences between these tumors. Experimental Design: Gene expression profiles of 75 cancers (endometrioid, serous, and clear cell) of the ovary and endometrium, five renal clear cell cancers, and seven normal epithelial brushings were determined using a 11,000-element cDNA array. All images were analyzed using BRB ArrayTools. Validation was done using real-time PCR on select genes and immunohistochemical staining. Results: Comparison across endometrial and ovarian cancers and serous and endometrioid tumors showed expression patterns reflecting their organ of origin. Clear cell tumors, however, showed remarkably similar expression patterns regardless of their origin, even when compared with renal clear cell samples. A set of 43 genes was common to comparisons of each of the three histologic subtypes of ovarian cancer with normal ovarian surface epithelium. Conclusions: The comparison of the gene expression profiles of endometrioid and serous subtypes of ovarian and endometrial cancer are largely unique to the combination of a particular subtype in a specific organ. In contrast, clear cell cancers show a remarkable similarity in gene expression profiles across organs (including kidney) and could not be statistically distinguished. © 2005 American Association for Cancer Research. |
| Keywords: | immunohistochemistry; controlled study; human tissue; human cell; histopathology; endometrioid carcinoma; endometrial neoplasms; endometrium cancer; ovarian neoplasms; ovary cancer; cluster analysis; gene expression profiling; kidney neoplasms; gene expression regulation, neoplastic; carcinoma, renal cell; oligonucleotide array sequence analysis; tumor suppressor protein p53; real time polymerase chain reaction; dna microarray; clear cell carcinoma; adenocarcinoma, clear cell; cystadenocarcinoma, serous; cancer classification; kidney cancer; computer program; carcinoma, endometrioid; serous carcinoma; complementary dna; histologic subtype; genetic difference; genetic similarity |
| Journal Title: | Clinical Cancer Research |
| Volume: | 11 |
| Issue: | 18 |
| ISSN: | 1078-0432 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2005-09-15 |
| Start Page: | 6422 |
| End Page: | 6430 |
| Language: | English |
| DOI: | 10.1158/1078-0432.ccr-05-0508 |
| PUBMED: | 16166416 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 130" - "Export Date: 24 October 2012" - "CODEN: CCREF" - "Source: Scopus" |
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