RNA silencing suppressor p21 of Beet yellows virus forms an RNA binding octameric ring structure Journal Article
| Authors: | Ye, K.; Patel, D. J. |
| Article Title: | RNA silencing suppressor p21 of Beet yellows virus forms an RNA binding octameric ring structure |
| Abstract: | Many plant viruses encode proteins that suppress the antiviral RNA silencing response mounted by the host. The suppressors p19 from tombusvirus and p21 from Beet yellows virus appear to block silencing by directly binding siRNA, a critical mediator in the process. Here, we report the crystal structure of p21, which reveals an octameric ring architecture with a large central cavity of 90 Å diameter. The all α-helical p21 monomer consists of N- and C-terminal domains that associate with their neighboring counterparts through symmetric head-to-head and tail-to-tail interactions. A putative RNA binding surface is identified in the conserved, positive-charged inner surface of the ring. In contrast to the specific p19-siRNA duplex interaction, p21 is a general nucleic acid binding protein, interacting with 21 nt or longer single- and double-stranded RNAs in vitro. This study reveals an RNA binding structure adopted by the p21 silencing suppressor. ©2005 Elsevier Ltd All rights reserved. |
| Keywords: | nonhuman; protein domain; carboxy terminal sequence; rna, small interfering; rna interference; protein interaction; rna; rna-binding proteins; amino acid sequence; molecular sequence data; amino terminal sequence; nucleotide sequence; crystal structure; protein structure, tertiary; protein p19; nucleic acid conformation; protein structure; protein p21; rna binding; double stranded rna; viral proteins; crystallography; nucleic acid binding protein; closterovirus; beet yellows virus; tombusvirus |
| Journal Title: | Structure |
| Volume: | 13 |
| Issue: | 9 |
| ISSN: | 0969-2126 |
| Publisher: | Cell Press |
| Date Published: | 2005-09-01 |
| Start Page: | 1375 |
| End Page: | 1384 |
| Language: | English |
| DOI: | 10.1016/j.str.2005.06.017 |
| PUBMED: | 16154094 |
| PROVIDER: | scopus |
| PMCID: | PMC4689306 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 28" - "Export Date: 24 October 2012" - "CODEN: STRUE" - "Molecular Sequence Numbers: GENBANK: AAC40863, AAC55667, NP_041877, NP_042870, NP_835252, YP224095;" - "Source: Scopus" |
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