A direct interaction between the RAG2 C terminus and the core histones is required for efficient V(D)J recombination Journal Article
| Authors: | West, K. L.; Singha, N. C.; De Ioannes, P.; Lacomis, L.; Erdjument-Bromage, H.; Tempst, P.; Cortes, P. |
| Article Title: | A direct interaction between the RAG2 C terminus and the core histones is required for efficient V(D)J recombination |
| Abstract: | V(D)J recombination is a tightly controlled process of somatic recombination whose regulation is mediated in part by chromatin structure. Here, we report that RAG2 binds directly to the core histone proteins. The interaction with histones is observed in developing lymphocytes and within the RAG1/RAG2 recombinase complex in a manner that is dependent on the RAG2 C terminus. Amino acids within the plant homeo domain (PHD)-like domain as well as a conserved acidic stretch of the RAG2 C terminus that is considered to be a linker region are important for this interaction. Point mutations that disrupt the RAG2-histone association inhibit the efficiency of the V(D)J recombination reaction at the endogenous immunoglobulin locus, with the most dramatic effect in the V to DJ H rearrangement. Copyright ©2005 by Elsevier Inc. |
| Keywords: | controlled study; human cell; dna-binding proteins; t lymphocyte; dna recombination; animals; mice; carboxy terminal sequence; cell line; protein binding; gene locus; immunoglobulin; protein interaction; homeodomain proteins; b lymphocyte; nuclear proteins; gene rearrangement; amino acid sequence; molecular sequence data; thymus gland; chromatin; recombination, genetic; peptide fragments; protein structure, tertiary; rag2 protein; point mutation; vdj recombinases; lymphocyte; histones; immunoglobulins; amino acid analysis; lymphoid cell; rabbits |
| Journal Title: | Immunity |
| Volume: | 23 |
| Issue: | 2 |
| ISSN: | 1074-7613 |
| Publisher: | Cell Press |
| Date Published: | 2005-08-01 |
| Start Page: | 203 |
| End Page: | 212 |
| Language: | English |
| DOI: | 10.1016/j.immuni.2005.07.004 |
| PUBMED: | 16111638 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 41" - "Export Date: 24 October 2012" - "CODEN: IUNIE" - "Source: Scopus" |
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