Genetic and functional characterization of isolated stromal cell lines from the aorta-gonado-mesonephros region Conference Paper
| Authors: | Weisel, K. C.; Moore, M. A. S. |
| Title: | Genetic and functional characterization of isolated stromal cell lines from the aorta-gonado-mesonephros region |
| Conference Title: | 5th International Symposium and Workshop on Hematopoietic Stem Cells |
| Abstract: | The hematopoietic system interacts with a supportive stromal environment allowing maintenance and differentiation of hematopoietic stem cells (HSCs). The aorta-gonado-mesonephros (AGM) region serves as a unique embryonic microenvironment, generating the first adult repopulating HSCs in the mouse embryo. To eludicate factors involved in hematopoietic support and induction of hematopoietic differentiation, we isolated more than 100 stromal cell clones derived from the AGM region of embryonic day (E) 10.5 mouse embryos for functional and genetic analysis. Selected isolated AGM stromal cell lines are highly efficient in supporting maintenance and expansion of mouse and human hematopoietic stem and progenitor cells. In addition, we can demonstrate for the first time that AGM stromal cell lines are also potent inducers of hematopoietic differentiation of murine embryonic stem cells. Stromal gene array analysis has identified genes that could play a role in hematopoietic support. © 2005 New York Academy of Sciences. |
| Keywords: | controlled study; human cell; nonhuman; conference paper; genetic analysis; animal cell; mouse; animals; mice; cell function; embryo; cell line; embryo development; cell differentiation; stem cell; hematopoietic system; cell isolation; hematopoietic stem cells; murinae; hematopoietic stem cell; cell clone; ex vivo study; stromal cells; gonad; gonads; coculture techniques; aorta; mesonephros; aorta-gonado-mesonephros region; ex vivo expansion; murine es cells; stromal coculture; aorta gonado mesonephros region; stromal cell line |
| Journal Title | Annals of the New York Academy of Sciences |
| Volume: | 1044 |
| Conference Dates: | 2004 Sept 16-18 |
| Conference Location: | Tübingen, Germany |
| ISBN: | 0077-8923 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2005-06-01 |
| Start Page: | 51 |
| End Page: | 59 |
| Language: | English |
| DOI: | 10.1196/annals.1349.007 |
| PUBMED: | 15958697 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 6" - "Export Date: 24 October 2012" - "CODEN: ANYAA" - "Source: Scopus" |
