Migration and differentiation of neural precursors derived from human embryonic stem cells in the rat brain Journal Article

Authors: Tabar, V.; Panagiotakos, G.; Greenberg, E. D.; Chan, B. K.; Sadelain, M.; Gutin, P. H.; Studer, L.
Article Title: Migration and differentiation of neural precursors derived from human embryonic stem cells in the rat brain
Abstract: Human embryonic stem (hES) cells provide a potentially unlimited cell source for regenerative medicine. Recently, differentiation strategies were developed to direct hES cells towards neural fates in vitro. However, the interaction of hES cell progeny with the adult brain environment remains unexplored. Here we report that hES cell-derived neural precursors differentiate into neurons, astrocytes and oligodendrocytes in the normal and lesioned brain of young adult rats and migrate extensively along white matter tracts. The differentiation and migration behavior of hES cell progeny was region specific. The hES cell-derived neural precursors integrated into the endogenous precursor pool in the subventricular zone, a site of persistent neurogenesis. Like adult neural stem cells, hES cell-derived precursors traveled along the rostral migratory stream to the olfactory bulb, where they contributed to neurogenesis. We found no evidence of cell fusion, suggesting that hES cell progeny are capable of responding appropriately to host cues in the subventricular zone.
Keywords: controlled study; nonhuman; methodology; letter; animal; cytology; animals; animal tissue; cells, cultured; embryonic stem cell; cell line; animal experiment; astrocyte; cell motion; stem cell transplantation; cell differentiation; neurons; in vitro study; biotechnology; physiology; animalia; stem cell; cell culture; brain; rat; cell migration; cell movement; stem cells; rats; rats, sprague-dawley; nervous system development; neurogenesis; nerve cell; medicine; nerve regeneration; sprague dawley rat; white matter; neurology; olfactory bulb; cell fusion; cells; oligodendroglia; human embryonic stem (hes) cells
Journal Title: Nature Biotechnology
Volume: 23
Issue: 5
ISSN: 1087-0156
Publisher: Nature Publishing Group  
Date Published: 2005-05-01
Start Page: 601
End Page: 606
Language: English
DOI: 10.1038/nbt1088
PUBMED: 15852001
PROVIDER: scopus
Notes: --- - "Cited By (since 1996): 91" - "Export Date: 24 October 2012" - "CODEN: NABIF" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Viviane S Tabar
    191 Tabar
  2. Philip H Gutin
    163 Gutin
  3. Lorenz Studer
    186 Studer
  4. Michel W J Sadelain
    543 Sadelain
  5. Bill Chan
    4 Chan