Association of free-prostate specific antigen subfractions and human glandular kallikrein 2 with volume of benign and malignant prostatic tissue Journal Article
| Authors: | Steuber, T.; Niemelä, P.; Haese, A.; Pettersson, K.; Erbersdobler, A.; Chun, K. H. F.; Graefen, M.; Kattan, M. W.; Huland, H.; Lilja, H. |
| Article Title: | Association of free-prostate specific antigen subfractions and human glandular kallikrein 2 with volume of benign and malignant prostatic tissue |
| Abstract: | BACKGROUND. We investigated the association of different subfractions of prostate specific antigen (PSA) and human glandular kallikrein 2 (hK2), such as total PSA (tPSA), complexed PSA (cPSA), free PSA (fPSA), "single-chain Intact fPSA" (fPSA-I), "multi-chain nicked fPSA" (fPSA-N), and total hK2 with volumes of total prostate gland, transition zone (tz), and prostate cancer (PCa) tissue in patients with benign and malignant prostatic disease. METHODS. Serum samples were collected from men with negative biopsy (n = 164) and PCa (n = 252). Total and fPSA were measured using a commercially immunoassay. We measured hK2 and fPSA-I by previously reported in-house research assays specific for hK2 and single-chain, non-cleaved fPSA, respectively. Levels of fPSA-N (=fPSA-fPSA-I) and cPSA (=tPSA-fPSA) were calculated. Total prostate and tz volume were measured using transrectal ultrasound (TRUS); PCa volume was calculated using a computer assisted volumetric program. Association with tz and cancer volumes (CaVols) was performed by linear regression analysis. RESULTS. All PSA subfractions and hK2 were associated with tz volume in multivariable linear regression analysis. Only hK2, fPSA, and fPSA-N were significantly associated with CaVol in multivariable analysis, fPSA-I seemed to be cancer related. CONCLUSIONS. The multi-chain fPSA-N subfractions of fPSA may be a valuable predictor of both benign prostate hyperplasia (BPH) and CaVol that is likely to be more useful in predicting tz volumes than CaVols. fPSA-I may provide information on cancer without being influenced by the presence of BPH. © 2004 Wiley-Liss, Inc. |
| Keywords: | adult; human tissue; aged; middle aged; unclassified drug; major clinical study; cancer diagnosis; prostate specific antigen; biological markers; diagnosis, differential; biopsy; prostate cancer; gleason score; prostate-specific antigen; prostatic neoplasms; statistical analysis; blood sampling; prostate biopsy; kallikrein; predictive value of tests; immunoassay; prostate hypertrophy; prostatic hyperplasia; regression analysis; serum; prostate disease; human kallikrein 2; tissue kallikreins; benign prostate hyperplasia; free-prostate specific antigen; free-prostate specific antigen subforms; free prostate specific antigen |
| Journal Title: | Prostate |
| Volume: | 63 |
| Issue: | 1 |
| ISSN: | 0270-4137 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2005-04-01 |
| Start Page: | 13 |
| End Page: | 18 |
| Language: | English |
| DOI: | 10.1002/pros.20156 |
| PUBMED: | 15378521 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 17" - "Export Date: 24 October 2012" - "CODEN: PRSTD" - "Source: Scopus" |
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