Are zinc-finger domains of protein kinase C dynamic structures that unfold by lipid or redox activation? Journal Article
| Authors: | Zhao, F.; Ilbert, M.; Varadan, R.; Cremers, C. M.; Hoyos, B.; Acin-Perez, R.; Vinogradov, V.; Cowburn, D.; Jakob, U.; Hammerling, U. |
| Article Title: | Are zinc-finger domains of protein kinase C dynamic structures that unfold by lipid or redox activation? |
| Abstract: | Protein kinase C (PKC) is activated by lipid second messengers or redox action, raising the question whether these activation modes involve the same or alternate mechanisms. Here we show that both lipid activators and oxidation target the zinc-finger domains of PKC, suggesting a unifying activation mechanism. We found that lipid agonist-binding or redox action leads to zinc release and disassembly of zinc fingers, thus triggering large-scale unfolding that underlies conversion to the active enzyme. These results suggest that PKC zinc fingers, originally considered purely structural devices, are in fact redox-sensitive flexible hinges, whose conformation is controlled both by redox conditions and lipid agonists. © 2011, Mary Ann Liebert, Inc. |
| Keywords: | nonhuman; animal cell; lipid; protein binding; enzyme activation; immunoblotting; protein kinase c; oxidative stress; oxidation; zinc finger motif; phosphatidylserine; redox stress |
| Journal Title: | Antioxidants & Redox Signaling |
| Volume: | 14 |
| Issue: | 5 |
| ISSN: | 1523-0864 |
| Publisher: | Mary Ann Liebert, Inc |
| Date Published: | 2011-03-01 |
| Start Page: | 757 |
| End Page: | 766 |
| Language: | English |
| DOI: | 10.1089/ars.2010.3773 |
| PROVIDER: | scopus |
| PMCID: | PMC3030452 |
| PUBMED: | 21067413 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 4 March 2011" - "CODEN: ARSIF" - "Source: Scopus" |
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