The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells Journal Article
| Authors: | Wallace, S. W.; Magalhaes, A.; Hall, A. |
| Article Title: | The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells |
| Abstract: | Rho GTPases regulate multiple signaling pathways to control a number of cellular processes during epithelial morphogenesis. To investigate the downstream pathways through which Rho regulates epithelial apical junction formation, we screened a small interfering RNA (siRNA) library targeting 28 known Rho target proteins in 16HBE human bronchial epithelial cells. This led to the identification of the serine-threonine kinase PRK2 (protein kinase C-related kinase 2, also called PKN2). Depletion of PRK2 does not block the initial formation of primordial junctions at nascent cell-cell contacts but does prevent their maturation into apical junctions. PRK2 is recruited to primordial junctions, and this localization depends on its C2-like domain. Rho binding is essential for PRK2 function and also facilitates PRK2 recruitment to junctions. Kinase-dead PRK2 acts as a dominant-negative mutant and prevents apical junction formation. We conclude that PRK2 is recruited to nascent cell-cell contacts through its C2-like and Rho-binding domains and promotes junctional maturation through a kinase-dependent pathway. Copyright © 2011, American Society for Microbiology. All Rights Reserved. |
| Keywords: | unclassified drug; human cell; protein function; small interfering rna; protein binding; cell junction; bronchus mucosa; protein kinase; enzyme localization; protein kinase c related kinase 2; rho factor |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 31 |
| Issue: | 1 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2011-01-01 |
| Start Page: | 81 |
| End Page: | 91 |
| Language: | English |
| DOI: | 10.1128/mcb.01001-10 |
| PROVIDER: | scopus |
| PMCID: | PMC3019857 |
| PUBMED: | 20974804 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 4 March 2011" - "CODEN: MCEBD" - "Source: Scopus" |
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