Nonrandom cell-cycle timing of a somatic chromosomal translocation: The t(X;17) of alveolar soft-part sarcoma occurs in G2 Journal Article
| Authors: | Huang, H. Y.; Lui, M. Y.; Ladanyi, M. |
| Article Title: | Nonrandom cell-cycle timing of a somatic chromosomal translocation: The t(X;17) of alveolar soft-part sarcoma occurs in G2 |
| Abstract: | The cell-cycle timing of somatic chromosomal translocations in cancer remains poorly understood but may be relevant to their etiology and the mechanism of their formation. Alveolar soft-part sarcoma (ASPS) is a rare malignant soft-tissue tumor of uncertain lineage that provides an opportunity to address this question. The great majority of ASPSs have relatively simple near-diploid karyotypes characterized by an unbalanced der(17)t(X;17)(p11.2;q25) , resulting in nonreciprocal fusion of TFE3 with ASPSCR1 (a.k.a. ASPL), with consequent net gain of Xp11.2→pter and loss of 17q25→qter. The presence of a normal X along with the der(17)t(X;17) in ASPSs that occur in men has been well described in previous cytogenetic reports and is most readily explained by a translocation in the G2 phase of the cell cycle. To establish whether formation in G2 is a general feature of the t(X;17), we examined polymorphic loci in Xp11.2→qter in ASPS from 9 women, including 7 with an unbalanced t(X;17). Our analysis showed that all 7 displayed retention of heterozygosity at all informative markers on Xp11.2→qter, supporting preferential formation of the t(X;17) in the G2 phase of the cell cycle. Given that the two derivative chromosomes of a translocation in G2 would be expected to segregate together half the time, the predominance of an unbalanced der(17)t(X;17) also raises the possibility of a selective advantage in ASPS cells for gain of Xp11.2→pter or loss of 17q25.3→qter or retention of an active copy of TFE3. © 2005 Wiley-Liss, Inc. |
| Keywords: | adolescent; adult; child; human tissue; preschool child; school child; middle aged; sarcoma; oncogene; heterozygosity; nucleotide sequence; gene fusion; chromosome translocation; chromosomes, human, x; translocation, genetic; cell cycle g2 phase; chromosome loss; karyotype; chromosome 17q; diploidy; chromosome segregation; genetic polymorphism; g2 phase; alveolar soft part sarcoma; tfe3 gene; chromosome xp; chromosomes, human, pair 17; aspscr1 gene |
| Journal Title: | Genes Chromosomes and Cancer |
| Volume: | 44 |
| Issue: | 2 |
| ISSN: | 1045-2257 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 2005-10-01 |
| Start Page: | 170 |
| End Page: | 176 |
| Language: | English |
| DOI: | 10.1002/gcc.20229 |
| PUBMED: | 15952162 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 8" - "Export Date: 24 October 2012" - "CODEN: GCCAE" - "Molecular Sequence Numbers: GENBANK: AC124283, AC124287" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work