Anesthetic-induced alteration in expression of myocardial calcium cycling proteins Journal Article
| Authors: | Heerdt, P. M.; The, A. H. S.; Markov, N. P. |
| Article Title: | Anesthetic-induced alteration in expression of myocardial calcium cycling proteins |
| Abstract: | Objective: The purpose of this study was to test the hypothesis that anesthesia alone or in combination with high FIO2 alters expression of the myocardial calcium cycling proteins, sarcoplasmic endoreticular calcium adenosine triphosphatase subtype 2a (SERCA2a), and the sarcolemmal sodium-calcium exchanger (NCX). Design: Multigroup comparison of protein expression using analysis of variance. Setting: University research laboratory. Subjects: Twenty-seven New Zealand white rabbits. Interventions: After sedation and the induction of anesthesia, animals underwent either tracheal intubation and ventilation for 5 hours with 1.0% end-tidal halothane in oxygen (HAL-O2, n = 5) or air (HAL-air, n = 5) or time-control recovery while spontaneously breathing oxygen (TC-O2, n = 5) or air (TC-air, n = 5) for 5 hours. Halothane dose was based on pilot data from 2 rabbits. Animals were then sacrificed, and the hearts were removed for Western blot analysis. Data were normalized to those from a group of rabbits immediately sacrificed (n = 5) without any prior treatment. Measurements and Main Results: In comparison to their respective time controls, SERCA2a was decreased 23 % in both HAL-air and HAL-O2 groups, whereas NCX was increased 34% and 122%, respectively. Expression was distinctly different between HAL-air and HAL-O2 for both SERCA2a (p = 0.009) and NCX (p < 0.001), indicating an influence of high FIO2. Similarly, SERCA2a in the TC-O2 group was reduced 25% relative to the TC-air group. Conclusion: Halothane alters the expression of myocardial calcium cycling proteins, and this effect is potentiated by high FIO2. These data offer the broad conclusion that perioperative interventions may influence the study of myocardial molecular remodeling and suggest the possibility of anesthetic-induced myocardial molecular remodeling. © 2005 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; protein expression; carrier protein; unclassified drug; nonhuman; animals; animal tissue; oxygen; sedation; animal experiment; time factors; blotting, western; western blotting; calcium-binding proteins; analysis of variance; myocardium; anesthesia; blood gas analysis; rabbits; breathing; rabbit; endotracheal intubation; intubation, intratracheal; anesthesia induction; sarcoplasmic reticulum calcium-transporting atpases; calcium-transporting atpases; serca; halothane; anesthetics, inhalation; remodeling; sodium-calcium exchanger; sarcoplasmic endoreticular calcium adenosine triphosphatase subtype 2a; sodium calcium exchange protein; assisted ventilation |
| Journal Title: | Journal of Cardiothoracic and Vascular Anesthesia |
| Volume: | 19 |
| Issue: | 3 |
| ISSN: | 1053-0770 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2005-06-01 |
| Start Page: | 340 |
| End Page: | 344 |
| Language: | English |
| DOI: | 10.1053/j.jvca.2005.02.007 |
| PUBMED: | 16130061 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 24 October 2012" - "CODEN: JCVAE" - "Source: Scopus" |
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