Different strategies for carboxyl-terminal domain (CTD) recognition by serine 5-specific CTD phosphatases Journal Article
| Authors: | Hausmann, S.; Koiwa, H.; Krishnamurthy, S.; Hampsey, M.; Shuman, S. |
| Article Title: | Different strategies for carboxyl-terminal domain (CTD) recognition by serine 5-specific CTD phosphatases |
| Abstract: | The phosphorylated carboxyl-terminal domain (CTD) of RNA polymerase II, consisting of ( 1YSPTSPS 7) n heptad repeats, encodes information about the state of the transcriptional apparatus that can be conveyed to factors that regulate mRNA synthesis and processing. Here we describe how the CTD code is read by two classes of protein phosphatases, plant CPLs and yeast Ssu72, that specifically dephosphorylate Ser 5 in vitro. The CPLs and Ssu72 recognize entirely different positional cues in the CTD primary structure. Whereas the CPLs rely on Tyr 1 and Pro 3 located on the upstream side of the Ser 5-PO 4 target site, Ssu72 recognizes Thr 4 and Pro 6 flanking the target Ser 5-PO 4 plus the downstream Tyr 1 residue of the adjacent heptad. We surmise that the reading of the CTD code does not obey uniform rules with respect to the location and phasing of specificity determinants. Thus, CTD code, like the CTD structure, is plastic. © by The American Society for Biochemistry and Molecular Biology, Inc. |
| Keywords: | unclassified drug; nonhuman; serine; carboxy terminal sequence; transcription, genetic; in vitro study; tyrosine; transcription factors; rna; rna-binding proteins; biosynthesis; enzyme phosphorylation; transcription regulation; amino acid sequence; saccharomyces cerevisiae; molecular recognition; carrier proteins; recombinant proteins; substrate specificity; protein structure, tertiary; protein dephosphorylation; yeast; saccharomyces cerevisiae proteins; enzyme kinetics; molecular biology; rna processing; enzyme specificity; biochemistry; rna polymerase ii; enzymes; lysine; fungal protein; proline; phosphoprotein phosphatase; arabidopsis; arabidopsis proteins; messenger rna synthesis; mrna synthesis; codes (standards); carboxyl-terminal domain (ctd); ctd phosphatadases; protein cpl1; protein cpl2; protein ssu72 |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 280 |
| Issue: | 45 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2005-11-11 |
| Start Page: | 37681 |
| End Page: | 37688 |
| Language: | English |
| DOI: | 10.1074/jbc.M505292200 |
| PUBMED: | 16148005 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 17" - "Export Date: 24 October 2012" - "CODEN: JBCHA" - "Source: Scopus" |
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