Cancer-testis genes are coordinately expressed and are markers of poor outcome in non-small cell lung cancer Journal Article


Authors: Gure, A. O.; Chua, R.; Williamson, B.; Gonen, M.; Ferrera, C. A.; Gnjatic, S.; Ritter, G.; Simpson, A. J. G.; Chen, Y. T.; Old, L. J.; Altorki, N. K.
Article Title: Cancer-testis genes are coordinately expressed and are markers of poor outcome in non-small cell lung cancer
Abstract: Purpose: Cancer-testis genes mapping to the X chromosome have common expression patterns and show similar responses to modulators of epigenetic mechanisms. We asked whether cancertestis gene expression occurred coordinately, and whether it correlated with variables of disease and clinical outcome of non - small cell lung cancer (NSCLC). Experimental Design: Tumors from 523 NSCLC patients undergoing surgery were evaluated for the expression of nine cancer-testis genes (NY-ESO-1, LAGE-1, MAGE-A1, MAGE-A3, MAGE-A4, MAGE-A10, CT7/MAGE-C1, SSX2, and SSX4) by semiquantitative PCR. Clinical data available for 447 patients were used to correlate cancer-testis expression to variables of disease and clinical outcome. Results: At least one cancer-testis gene was expressed by 90% of squamous carcinoma, 62% of bronchioloalveolar cancer, and 67% of adenocarcinoma samples. Statistically significant coexpression was observed for 34 of the 36 possible cancer-testis combinations. Cancer-testis gene expression, either cumulatively or individually, showed significant associations with male sex, smoking history, advanced tumor, nodal and pathologic stages, pleural invasion, and the absence of ground glass opacity. Cox regression analysis revealed the expression of NY-ESO-1 and MAGE-A3 as markers of poor prognosis, independent of confounding variables for adenocarcinoma of the lung. Conclusions: Cancer-testis genes are coordinately expressed in NSCLC, and their expression is associated with advanced disease and poor outcome. © 2005 American Association for Cancer Research.
Keywords: adult; human tissue; middle aged; survival analysis; major clinical study; advanced cancer; polymerase chain reaction; gene; gene expression; lung non small cell cancer; carcinoma, non-small-cell lung; lung neoplasms; tumor markers, biological; membrane proteins; smoking; cancer invasion; gene mapping; gene expression regulation, neoplastic; correlation analysis; antigens, neoplasm; evaluation; reverse transcriptase polymerase chain reaction; epigenetics; quantitative analysis; x chromosome; chromosomes, human, x; lung alveolus cell carcinoma; gender; regression analysis; testis; cancer testis gene; lage 1 gene; ct7 gene; mage a1 gene; mage a10 gene; mage a3 gene; mage a4 gene; ny eso 1 gene; ssx2 gene; ssx4 gene
Journal Title: Clinical Cancer Research
Volume: 11
Issue: 22
ISSN: 1078-0432
Publisher: American Association for Cancer Research  
Date Published: 2005-11-15
Start Page: 8055
End Page: 8062
Language: English
DOI: 10.1158/1078-0432.ccr-05-1203
PUBMED: 16299236
PROVIDER: scopus
DOI/URL:
Notes: --- - "Cited By (since 1996): 100" - "Export Date: 24 October 2012" - "CODEN: CCREF" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Mithat Gonen
    713 Gonen
  2. Ali O Gure
    29 Gure
  3. Andrew John Simpson
    31 Simpson
  4. Sacha Gnjatic
    111 Gnjatic
  5. Gerd Ritter
    158 Ritter
  6. Ramon Chua
    19 Chua
  7. Lloyd J Old
    385 Old