Cell proliferation of cultured human cancer cells are affected by the elevated tumor pressures that exist in vivo Journal Article
| Authors: | DiResta, G. R.; Nathan, S. S.; Manoso, M. W.; Casas-Ganem, J.; Wyatt, C.; Kubo, T.; Boland, P. J.; Athanasian, E. A.; Miodownik, J.; Gorlick, R.; Healey, J. H. |
| Article Title: | Cell proliferation of cultured human cancer cells are affected by the elevated tumor pressures that exist in vivo |
| Abstract: | Elevated interstitial fluid pressure (IFP) is observed in most solid tumors. However, the study of the cellular processes of tumors and the development of chemotherapy are routinely studied using in vitro culture systems at atmospheric pressure. Using a new pressurized cell culture system, we investigated the influence of hydrostatic pressure on population dynamics of three primary osteosarcoma (HOS, U2OS, SaOS2) and two metastatic tumor cell lines (MCF7 breast, H1299 lung) that invade bone. Values of IFP in normal human bone and muscle, and in osteosarcoma tumors obtained during their surgical biopsy established the hydrostatic pressure range for the in vitro cell studies. The IFP values were obtained from a retrospective review of patient records. IFP from confirmed osteosarcoma was 35.9± 16.2 mmHg. Tumor IFP was significantly higher than muscle IFP (p < 0.001) and bone IFP (p < 0.003). The in vitro study measured the cell-line proliferation using hydrostatic pressures of 0, 20, 50 and 100 mmHg. The findings suggest that hydrostatic pressure either increases or decreases tumor proliferation rates depending on cell type. Furthermore, cell death was not associated with apoptosis. © 2005 Biomedical Engineering Society. |
| Keywords: | osteosarcoma; pathophysiology; chemotherapy; neoplasm; neoplasms; cell proliferation; cell death; breast cancer; lung cancer; cell line, tumor; biopsy; physiology; cell culture; tumors; tumor cell line; population dynamics; cells; musculoskeletal system; hydrostatic pressure; interstitial fluid pressure; logistic equation; interstitial fluid pressure (ifp); atmospheric pressure |
| Journal Title: | Annals of Biomedical Engineering |
| Volume: | 33 |
| Issue: | 9 |
| ISSN: | 0090-6964 |
| Publisher: | Springer |
| Date Published: | 2005-09-01 |
| Start Page: | 1270 |
| End Page: | 1280 |
| Language: | English |
| DOI: | 10.1007/s10439-005-5732-9 |
| PROVIDER: | scopus |
| PUBMED: | 16133932 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 17" - "Export Date: 24 October 2012" - "CODEN: ABMEC" - "Source: Scopus" |
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MSK Authors
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7Kubo -
13
Casas-Ganem -
160Boland -
75Athanasian -
121
Gorlick -
48
Di Resta -
9
Manoso -
10
Nathan -
547Healey -
1
Wyatt
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