Simultaneous haploinsufficiency of Pten and Trp53 tumor suppressor genes accelerates tumorigenesis in a mouse model of prostate cancer Journal Article


Authors: Couto, S. S.; Cao, M.; Duarte, P. C.; Banach-Petrosky, W.; Wang, S.; Romanienko, P.; Wu, H.; Cardiff, R. D.; Abate-Shen, C.; Cunha, G. R.
Article Title: Simultaneous haploinsufficiency of Pten and Trp53 tumor suppressor genes accelerates tumorigenesis in a mouse model of prostate cancer
Abstract: Tumor suppressor gene PTEN is important in the initiation and progression of human prostate carcinoma, whereas the role of TP53 remains controversial. Since Pten/Trp53 double conditional knockout mice show earlier onset and fast progression of prostate cancer when compared to Pten knockout mice, we asked whether heterozygosity of these two tumor suppressor genes was sufficient to accelerate prostatic tumorigenesis. To answer this question we examined prostatic lesion progression of Pten/Trp53 double heterozygous mice and a series of controls such as Pten heterozygous, Pten conditional knockout, Trp53 heterozygous and Trp53 knockout mice. Tissue recombination of adult prostatic epithelium coupled with embryonic rat seminal vesicle mesenchyme was used as a tool to stimulate prostatic epithelial proliferation. In our study, high-grade prostatic intraepithelial neoplasia (PIN) was found with high frequency at 8 weeks post-tissue recombination transplantation. PIN lesions in Pten/Trp53 double heterozygous mice were more severe than those seen in Pten heterozygous alone. Furthermore, morphologic features attributable to Pten or Trp53 loss appeared to be enhanced in double heterozygous tissues. LOH analysis of Pten and Trp53 in genomic DNA collected from high-grade PIN lesions in Pten heterozygous and Pten/Trp53 double heterozygous mice showed an intact wild-type allele for both genes in all samples examined. In conclusion, simultaneous heterozygosity of Pten and Trp53 accelerates prostatic tumorigenesis in this mouse model of prostate cancer independently of loss of heterozygosity of either gene. © 2008 International Society of Differentiation.
Keywords: controlled study; protein expression; nonhuman; cell proliferation; mouse; animals; mice; mice, knockout; animal tissue; mus; animal experiment; animal model; cell differentiation; pathology; heterozygote; protein p53; carcinogenesis; prostate cancer; prostatic neoplasms; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; pten phosphohydrolase; prostate epithelium; tumor suppressor protein p53; rats; prostatic intraepithelial neoplasia; rattus; akt; embryonic mesenchyme; mouse models; pten; tissue recombinants; trp53; tumor suppressor genes; seminal vesicle; disease models, animal; loss of heterozygosity
Journal Title: Differentiation
Volume: 77
Issue: 1
ISSN: 0301-4681
Publisher: International Society of Differentiation  
Date Published: 2009-01-01
Start Page: 103
End Page: 111
Language: English
DOI: 10.1016/j.diff.2008.09.010
PUBMED: 19281769
PROVIDER: scopus
PMCID: PMC2828345
DOI/URL:
Notes: --- - "Cited By (since 1996): 2" - "Export Date: 30 November 2010" - "CODEN: DFFNA" - "Source: Scopus"
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  1. Suzana S Couto
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