Discovery of hundreds of mirtrons in mouse and human small RNA data Journal Article

Authors: Ladewig, E.; Okamura, K.; Flynt, A. S.; Westholm, J. O.; Lai, E. C.
Article Title: Discovery of hundreds of mirtrons in mouse and human small RNA data
Abstract: Atypical miRNA substrates do not fit criteria often used to annotate canonical miRNAs, and can escape the notice of miRNA genefinders. Recent analyses expanded the catalogs of invertebrate splicing-derived miRNAs ("mirtrons"), but only a few tens of mammalian mirtrons have been recognized to date. We performed meta-analysis of 737 mouse and human small RNA data sets comprising 2.83 billion raw reads. Using strict and conservative criteria, we provide confident annotation for 237 mouse and 240 human splicing-derived miRNAs, the vast majority of which are novel genes. These comprise three classes of splicing-derived miRNAs in mammals: conventional mirtrons, 5′-tailed mirtrons, and 3′-tailed mirtrons. In addition, we segregated several hundred additional human and mouse loci with candidate (and often compelling) evidence. Most of these loci arose relatively recently in their respective lineages. Nevertheless, some members in each of the three mirtron classes are conserved, indicating their incorporation into beneficial regulatory networks. We also provide the first Northern validation for mammalian mirtrons, and demonstrate Dicer-dependent association of mature miRNAs from all three classes of mirtrons with Ago2. The recognition of hundreds of mammalian mirtrons provides a new foundation for understanding the scope and evolutionary dynamics of Dicer substrates in mammals. © 2012, Published by Cold Spring Harbor Laboratory Press.
Keywords: nonhuman; animals; mice; microrna; molecular dynamics; gene locus; genetic variability; intron; gene expression regulation; conserved sequence; molecular sequence data; sequence alignment; mammal; molecular recognition; base sequence; gene regulatory network; rna structure; micrornas; mammals; rna splicing; dicer; argonaute 2 protein; molecular sequence annotation; genetic loci; rna, small untranslated; mirtron; argonaute proteins
Journal Title: Genome Research
Volume: 22
Issue: 9
ISSN: 1088-9051
Publisher: Cold Spring Harbor Laboratory Press  
Date Published: 2012-09-01
Start Page: 1634
End Page: 1645
Language: English
DOI: 10.1101/gr.133553.111
PROVIDER: scopus
PMCID: PMC3431481
PUBMED: 22955976
Notes: --- - "Cited By (since 1996): 1" - "Export Date: 1 October 2012" - "CODEN: GEREF" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Eric C Lai
    135 Lai
  2. Alex S Flynt
    12 Flynt
  3. Katsutomo Okamura
    26 Okamura
  4. Erik Manfred Ladewig
    14 Ladewig